Korhonen Taina, Grauling-Halama Silke, Halama Niels, Silvennoinen-Kassinen Sylvi, Leinonen Maija, Saikku Pekka
Laboratory for Chlamydia and Respiratory Tract Bacteria, National Public Health Institute, PO Box 310, Fin-90101 Oulu, Finland.
J Immunol Methods. 2006 Dec 20;317(1-2):171-4. doi: 10.1016/j.jim.2006.07.029. Epub 2006 Nov 3.
Lipopolysaccharide (LPS) is an exdotoxin found in the outer membrane of gram-negative bacteria. In circulation, LPS is bound by LPS-binding protein (LBP), which participates in cell activation by transferring LPS to CD14 and Toll-like receptor 4. A high LPS concentration may give rise to an exaggerated immune response, which may lead to septic shock during septicemia. However, LBP also neutralizes and removes LPS by transferring it to plasma lipoproteins. Recently, the presence of an amino acid-changing polymorphism in the LBP gene was reported, which, in men, was associated with sepsis and its severity and with myocardial infarction. Here, we describe a new LightCycler real-time PCR method for genotyping this LBP C(1341)-->T (Leu(436)-->Phe) polymorphism. In our study population of 393 Finnish blood donors, the genotype frequencies were: 86% TT, 13% CT and 1% CC.
脂多糖（LPS）是一种存在于革兰氏阴性菌外膜中的外毒素。在血液循环中，LPS与LPS结合蛋白（LBP）结合，LBP通过将LPS转移至CD14和Toll样受体4参与细胞活化。高浓度的LPS可能引发过度免疫反应，在败血症期间可能导致感染性休克。然而，LBP也通过将LPS转移至血浆脂蛋白来中和并清除LPS。最近，有报道称LBP基因存在一种导致氨基酸改变的多态性，在男性中，该多态性与败血症及其严重程度以及心肌梗死有关。在此，我们描述了一种用于对这种LBP C(1341)-->T（Leu(436)-->Phe）多态性进行基因分型的新型LightCycler实时聚合酶链反应方法。在我们由393名芬兰献血者组成的研究群体中，基因型频率为：TT占86%，CT占13%，CC占1%。
