人类角质层中可变的角蛋白多肽谱。

Variable keratin polypeptide profile in human stratum corneum.

作者信息

Yaar M, Matoltsy G A, Gilchrest B A

机构信息

Cutaneous Gerontology Laboratory, USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111.

出版信息

J Dermatol Sci. 1990 Jul;1(4):261-8. doi: 10.1016/0923-1811(90)90118-w.

DOI:10.1016/0923-1811(90)90118-w

PMID:1710140

Abstract

Stratum corneum samples obtained from 46 members in three generations of seven families were analyzed for keratin pattern by gel electrophoresis. All these samples of apparently normal upper arm skin expressed the 55 kDa, 56.5 kDa, and 65 kDa keratin proteins; while only 28%, 20% and 48% of the samples expressed the 50 kDa, 58 kDa, and 67 kDa proteins, respectively. The keratin phenotype was identical in all members of two families (9 individuals) and variable in members of five other families (37 individuals), in whom the patterns were consistent with autosomal dominant inheritance. These results demonstrate inter-individual variations in stratum corneum keratin pattern and may reflect either polymorphism of genes coding for the various keratin polypeptides or a post transcriptional modification of mature keratins by proteolytic digestion.

摘要

从7个家族三代中的46名成员获取的角质层样本，通过凝胶电泳分析其角蛋白模式。所有这些看似正常的上臂皮肤样本均表达55 kDa、56.5 kDa和65 kDa的角蛋白；而分别只有28%、20%和48%的样本表达50 kDa、58 kDa和67 kDa的蛋白。两个家族（9名个体）的所有成员角蛋白表型相同，其他五个家族（37名个体）的成员角蛋白表型存在差异，其模式符合常染色体显性遗传。这些结果表明角质层角蛋白模式存在个体间差异，可能反映了编码各种角蛋白多肽的基因多态性，或成熟角蛋白经蛋白水解消化后的转录后修饰。