Kaczmarek Anna, Naganowska Barbara, Wolko Bogdan
Polish Academy of Sciences, Institute of Plant Genetics, Poznań, Poland.
Cell Mol Biol Lett. 2007;12(1):16-24. doi: 10.2478/s11658-006-0056-9. Epub 2006 Nov 13.
Two molecular cytogenetics methods, PRINS (primed in situ DNA labeling) and C-PRINS (cycling PRINS), were optimized for the physical mapping of several types of DNA sequences on the mitotic chromosomes of the narrow-leafed lupin (Lupinus angustifolius L.). The fragment of the FokI element from Vicia faba was localised by indirect PRINS reaction. Two other sequences, fragments of the coding sequences of L. luteus and of L. angustifolius, were localised by indirect C-PRINS. These techniques are faster and more sensitive than FISH, and they allowed the mapping of short DNA fragments. The data obtained shows that both types of PRINS are valuable tools for chromosome identification in lupin.
两种分子细胞遗传学方法,即引物原位DNA标记法(PRINS)和循环引物原位DNA标记法(C-PRINS),针对窄叶羽扇豆(Lupinus angustifolius L.)有丝分裂染色体上几种类型的DNA序列的物理定位进行了优化。通过间接PRINS反应对来自蚕豆的FokI元件片段进行了定位。另外两个序列,即黄羽扇豆和窄叶羽扇豆编码序列的片段,通过间接C-PRINS进行了定位。这些技术比荧光原位杂交(FISH)更快、更灵敏,并且能够对短DNA片段进行定位。所获得的数据表明,这两种类型的PRINS都是羽扇豆染色体鉴定的有价值工具。