通过荧光原位杂交对羽扇豆属植物中18S - 25S核糖体DNA和5S核糖体DNA进行物理图谱分析。
Physical mapping of 18S-25S rDNA and 5S rDNA in Lupinus via fluorescent in situ hybridization.
作者信息
Naganowska Barbara, Zielińska Anna
机构信息
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań Poland.
出版信息
Cell Mol Biol Lett. 2002;7(2B):665-70.
Double-target fluorescent in situ hybridization (FISH) was used to determine the genomic distribution of ribosomal RNA genes in five Lupinus species: L. cosentinii (2n=32), L. pilosus (2n=42), L. angustifolius (2n=40), L. luteus (2n=52) and L. mutabilis (2n=48). 18S-25S rDNA and 5S rDNA were used as probes. Some interspecific variation was observed in the number and size of the 18S-25S rDNA loci. All the studied species had one chromosome pair carrying 5S rDNA.
采用双靶点荧光原位杂交（FISH）技术，确定了5种羽扇豆属植物核糖体RNA基因的基因组分布，这5种植物分别是：科森蒂尼羽扇豆（2n = 32）、柔毛羽扇豆（2n = 42）、窄叶羽扇豆（2n = 40）、黄羽扇豆（2n = 52）和多变羽扇豆（2n = 48）。使用18S - 25S rDNA和5S rDNA作为探针。在18S - 25S rDNA位点的数量和大小上观察到一些种间变异。所有研究的物种都有一对携带5S rDNA的染色体。
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