Guo Hui-Fang, Fang Ji-Chao, Liu Bao-Sheng, Wang Jie-Ping, Zhong Wan-Fang, Wan Fang-Hao
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Pest Manag Sci. 2007 Jan;63(1):68-74. doi: 10.1002/ps.1316.
The enhancement of Spodoptera litura (F.) nucleopolyhedrovirus (SlNPV) activity using the chitin synthesis inhibitor chlorfluazuron was investigated. When tested against fifth-instar S. litura larvae, chlorfluazuron produced synergistic effects at doses of 0.05 and 0.025 microg per insect, and additive effects at doses of 0.1 and 0.2 microg. Furthermore, the time required for SlNPV to kill larvae was significantly reduced by chlorfluazuron at all doses tested. The activity and killing speed of Autographa californica (Spey) nucleopolyhedrovirus (AcMNPV) against third-instar Spodoptera exigua (Hübner) larvae were similarly improved by chlorfluazuron at a dose of 0.05 microg per larva. Furthermore, the growth of S. exigua was significantly retarded by chlorfluazuron. Environmental scanning electron microscopy (ESEM) showed that the peritrophic matrices (PMs) of S. litura exposed to chlorfluazuron alone, or the combination treatment, were markedly disrupted. Obvious ruptures on the outer surfaces of the PM were observed, which potentially facilitated the passage of virions through the matrix.
研究了使用几丁质合成抑制剂氟铃脲增强斜纹夜蛾核多角体病毒（SlNPV）活性的情况。在针对五龄斜纹夜蛾幼虫进行测试时，氟铃脲在每头昆虫0.05和0.025微克的剂量下产生协同效应，在0.1和0.2微克的剂量下产生相加效应。此外，在所有测试剂量下，氟铃脲均显著缩短了SlNPV杀死幼虫所需的时间。在每头幼虫0.05微克的剂量下，氟铃脲同样提高了苜蓿银纹夜蛾核多角体病毒（AcMNPV）对三龄甜菜夜蛾幼虫的活性和杀灭速度。此外，氟铃脲显著抑制了甜菜夜蛾的生长。环境扫描电子显微镜（ESEM）显示，单独暴露于氟铃脲或联合处理的斜纹夜蛾围食膜（PMs）明显受到破坏。观察到PM外表面有明显破裂，这可能有助于病毒粒子穿过该基质。
