Sever C E, Locker J
Department of Pathology, University of Pittsburgh School of Medicine, Pennsylvania 15261.
Mol Carcinog. 1991;4(2):138-44. doi: 10.1002/mc.2940040209.
cDNA probes for human retinoic acid receptors alpha and beta (RAR alpha and RAR beta) were modified for use as specific hybridization probes to study hepatocellular carcinomas (HCC) and cell lines, liver regeneration, and fetal development. RAR beta mRNA was detected at low levels in adult liver and rose markedly during the early phase of liver regeneration. RAR beta mRNA was present at very low levels in HCC and was not detected in fetal liver. In contrast, RAR alpha mRNA was present at low levels in normal liver, but showed a marked elevation in several HCCs and cell lines. Growth of cell lines was altered by retinoic acid (RA), but the effects could not be predicted by the levels of either RAR alpha or RAR beta mRNA. However, the response correlated with cell phenotype. Three cell lines with an adult phenotype (high albumin and low alpha-fetoprotein) were inhibited by RA, two undifferentiated lines showed moderate growth stimulation, and two of three cell lines that had high levels of alpha-fetoprotein were markedly stimulated by RA.
用于人类视黄酸受体α和β（RARα和RARβ）的cDNA探针经过修饰，用作特异性杂交探针，以研究肝细胞癌（HCC）和细胞系、肝脏再生及胎儿发育。在成年肝脏中可检测到低水平的RARβ mRNA，且在肝脏再生早期显著升高。RARβ mRNA在HCC中含量极低，在胎儿肝脏中未检测到。相比之下，RARα mRNA在正常肝脏中含量较低，但在一些HCC和细胞系中显著升高。视黄酸（RA）可改变细胞系的生长，但无法通过RARα或RARβ mRNA的水平预测其作用效果。然而，这种反应与细胞表型相关。三种具有成年表型（高白蛋白和低甲胎蛋白）的细胞系受到RA抑制，两种未分化的细胞系表现出适度的生长刺激，三个甲胎蛋白水平高的细胞系中有两个受到RA的显著刺激。
