Sun J B, Olsson T, Wang W Z, Xiao B G, Kostulas V, Fredrikson S, Ekre H P, Link H
Department of Neurology, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden.
Eur J Immunol. 1991 Jun;21(6):1461-8. doi: 10.1002/eji.1830210620.
The pathogenesis of multiple sclerosis (MS) could involve an autoimmune response to proteolipid protein (PLP). Immunization of experimental animals with this major myelin protein can lead to experimental allergic encephalomyelitis. To identify a possible role of PLP as target antigen in MS, we evaluated T cell immunity to PLP in blood and cerebrospinal fluid (CSF) from patients with MS and controls by counting cells which in response to PLP in short-term cultures secreted interferon-gamma. The PLP-specific B cell response was analyzed by counting cells secreting anti-PLP antibodies. PLP-reactive T cells were detected in blood of most MS patients (mean value 1 per 20,408 mononuclear cells), and at 41-fold higher numbers in CSF (mean 1 per 500 CSF cells). Anti-PLP IgG antibody-secreting cells were detected in blood from most MS patients (mean 1 per 30,303 cells), but such cells were 49-fold more frequent in CSF (mean 1 per 625 cells). PLP-reactive T and B cells were also detected in blood and CSF from control patients, but at much lower numbers. A strong and persistent autoimmune response to PLP as well as to other myelin proteins, enriched in CSF, is proposed to be pathogenetically important in MS.
多发性硬化症（MS）的发病机制可能涉及针对蛋白脂蛋白（PLP）的自身免疫反应。用这种主要的髓鞘蛋白对实验动物进行免疫可导致实验性变应性脑脊髓炎。为了确定PLP作为MS靶抗原的可能作用，我们通过对短期培养中对PLP有反应并分泌γ干扰素的细胞进行计数，评估了MS患者和对照组血液及脑脊液（CSF）中针对PLP的T细胞免疫。通过对分泌抗PLP抗体的细胞进行计数来分析PLP特异性B细胞反应。在大多数MS患者的血液中检测到了PLP反应性T细胞（平均每20408个单核细胞中有1个），而在脑脊液中的数量则高出41倍（平均每500个脑脊液细胞中有1个）。在大多数MS患者的血液中检测到了分泌抗PLP IgG抗体的细胞（平均每30303个细胞中有1个），但此类细胞在脑脊液中的频率高出49倍（平均每625个细胞中有1个）。在对照患者的血液和脑脊液中也检测到了PLP反应性T细胞和B细胞，但数量要少得多。有人提出，对PLP以及其他富含脑脊液的髓鞘蛋白产生强烈而持续的自身免疫反应在MS的发病机制中具有重要意义。
