Kontsek P, Borecký L, Novák M, Kontseková E, Máciková I
Institute of Virology, Slovak Academy of Sciences, Bratislava, Czech.
Immunology. 1991 May;73(1):8-11.
Three murine monoclonal antibodies (mAb) directed to distinct epitopes on recombinant human interferon (IFN)-alpha 1, and three mAb recognizing distinct epitopes on recombinant human interferon (IFN) alpha 1, and three mAb recognizing distinct epitopes on recombinant human IFN-alpha sc, were studied by IFN-neutralizing assays. The efficacy of neutralization of the anti-viral and the anti-proliferative activities of IFN-alpha 1, or IFN-alpha 2c, by the specific antibodies used, individually or in combination, were evaluated. In comparison with single mAb, the mixtures of three mAb against IFN-alpha 1 or three mAb against IFN-alpha 2c were capable of neutralizing more than 10-times larger amounts of IFN-alpha 1 and alpha 2c, respectively. The strong potentiation of the neutralization efficacy resulting from mixing different mAb was demonstrated by neutralization of the anti-viral as well as the anti-proliferative activities of both recombinant IFN. The neutralization experiments support the interpretation that the observed potentiation results from simultaneous interaction of anti-IFN mAb with different epitope specificity.
通过干扰素中和试验研究了三种针对重组人干扰素（IFN）-α1上不同表位的鼠单克隆抗体（mAb）、三种识别重组人干扰素（IFN）α1上不同表位的mAb以及三种识别重组人IFN-αsc上不同表位的mAb。评估了所使用的特异性抗体单独或联合使用时对IFN-α1或IFN-α2c的抗病毒和抗增殖活性的中和效力。与单克隆mAb相比，三种针对IFN-α1的mAb混合物或三种针对IFN-α2c的mAb混合物分别能够中和比IFN-α1和α2c多10倍以上的量。通过对两种重组干扰素的抗病毒和抗增殖活性进行中和，证明了混合不同mAb导致中和效力的强烈增强。中和实验支持这样的解释，即观察到的增强是由于抗IFN mAb与不同表位特异性同时相互作用所致。
