Kawade Y, Watanabe Y
Immunology. 1985 Nov;56(3):489-95.
Several monoclonal antibodies to human interferon-alpha and beta were examined quantitatively for neutralization of antigenically homogeneous interferons. The extent of neutralization increased with antibody concentration in each case with a rate considerably lower than in the case of conventional (polyclonal) antibodies, and there often appeared to be a limit to the maximum interferon titre that can be neutralized, even using very high antibody concentrations (there were no such limits with conventional antibodies). This suggests that the interferon in a 1:1 interferon-antibody complex in general retains some activity, to the degree characteristic of that antibody; namely, each monoclonal antibody is considered to have a characteristic efficacy of neutralization, rather than being either neutralizing or non-neutralizing in an all-or-one fashion. The antibody dose-dependence curves were interpreted to be governed by two independent parameters of the antibody: the efficacy of neutralization and the affinity. The former is reflected by the maximum interferon titre neutralizable by high antibody doses and the latter by the minimum antibody dose that can effect detectable neutralization. Thus, quantitative neutralization tests of monoclonal antibodies to an effector protein would give useful information for classifying them as to their affinities and as to whether the epitopes they recognize are important for the biological activity of the effector.
对几种抗人α-干扰素和β-干扰素的单克隆抗体进行了定量检测，以确定其对抗抗原性均一的干扰素的中和作用。在每种情况下，中和程度均随抗体浓度的增加而升高，但其速率远低于传统（多克隆）抗体，而且即使使用非常高的抗体浓度，通常似乎也存在可中和的最大干扰素滴度的极限（传统抗体不存在这种极限）。这表明，在1:1的干扰素-抗体复合物中的干扰素通常会保留一定活性，其程度具有该抗体的特征；也就是说，每种单克隆抗体被认为具有特征性的中和效力，而不是以全或无的方式要么具有中和作用要么不具有中和作用。抗体剂量依赖性曲线被解释为由抗体的两个独立参数决定：中和效力和亲和力。前者由高抗体剂量可中和的最大干扰素滴度反映，后者由可产生可检测到的中和作用的最小抗体剂量反映。因此，对效应蛋白的单克隆抗体进行定量中和试验，将为根据其亲和力以及它们识别的表位对效应蛋白的生物学活性是否重要来对它们进行分类提供有用信息。
