Tamura M, Yoshino T, Hattori K, Kawamura A, Nomura H, Imai N, Ono M
Fuji-Gotemba Research Laboratories, Chugai Pharmaceutical Company, Shizuoka, Japan.
Transplantation. 1991 Jun;51(6):1166-70. doi: 10.1097/00007890-199106000-00005.
We have investigated the effects of recombinant human granulocyte colony-stimulating factor (rG-CSF) on hemopoietic reconstitution after bone marrow transplantation (BMT) following lethal irradiation in mice. Mice received a daily administration of 10 micrograms/kg rG-CSF or control vehicle one through 21 days after BMT. Spleen colony-forming units (CFU-S), granulocyte-macrophage colony-forming units (CFU-GM), megakaryocyte colony-forming units (CFU-Meg), and erythroid burst-forming units (BFU-E) increased in both bone marrow and spleen of the rG-CSF-treated mice as compared with the control. This increase was evident during the administration period. In spite of the increase in the progenitor cells in bone marrow and spleen, only a recovery of neutrophils was accelerated in peripheral blood. Thus rG-CSF accelerated granulopoietic recovery in the BMT mice, with an enhanced recovery of the stem cells and the progenitors for erythrocytes and megakaryocytes. These results indicate the potential clinical usefulness of rG-CSF in the treatment of patients undergoing BMT.
我们研究了重组人粒细胞集落刺激因子（rG-CSF）对致死性照射后小鼠骨髓移植（BMT）后造血重建的影响。在BMT后的1至21天，小鼠每天接受10微克/千克的rG-CSF或对照载体。与对照组相比，rG-CSF处理的小鼠骨髓和脾脏中的脾集落形成单位（CFU-S）、粒细胞-巨噬细胞集落形成单位（CFU-GM）、巨核细胞集落形成单位（CFU-Meg）和红系爆式集落形成单位（BFU-E）均增加。这种增加在给药期间很明显。尽管骨髓和脾脏中的祖细胞有所增加，但外周血中仅中性粒细胞的恢复加速。因此，rG-CSF加速了BMT小鼠的粒细胞生成恢复，同时增强了红细胞和巨核细胞干细胞及祖细胞的恢复。这些结果表明rG-CSF在治疗接受BMT的患者方面具有潜在的临床应用价值。
