Kallinowski F, Friis R R, Van Roy F, Vaupel P
Dept. of Radiation Medicine, Mass. General Hospital, Harvard Medical School, Boston 02114.
Adv Exp Med Biol. 1990;277:907-16. doi: 10.1007/978-1-4684-8181-5_104.
In order to gain insight into mechanisms governing the development of tumor hypoxia, malignancies derived from spontaneously tumorigenic or ras-transformed cell lines were grown in nude mice. As a rule, tumors with ras oncogenes exhibited rapid growth rates and large areas with low pO2 readings even at small tumor sizes. The slow proliferation rate of a spontaneously tumorigenic cell line was consistent with more adequate tissue oxygen levels. In all lines, hypoxia was accentuated at larger tumor sizes. These results demonstrate that ras transformation can lead to accelerated proliferation rates and is then concomitant with the development of pronounced tumor hypoxia.
为了深入了解肿瘤缺氧发生发展的机制，将源自自发致瘤或ras转化细胞系的恶性肿瘤接种到裸鼠体内进行培养。通常，携带ras癌基因的肿瘤即使在体积较小时也表现出快速生长速度，且存在大片低氧分压读数区域。自发致瘤细胞系的增殖速度较慢，这与组织氧水平更充足相一致。在所有细胞系中，肿瘤体积较大时缺氧情况会加重。这些结果表明，ras转化可导致增殖速度加快，进而伴随着明显的肿瘤缺氧的发生。
