评估NTERA-2细胞作为研究人类胚胎干细胞模型的多能性和多谱系分化潜能。
Assessment of pluripotency and multilineage differentiation potential of NTERA-2 cells as a model for studying human embryonic stem cells.
作者信息
Pal Rajarshi, Ravindran Geeta
机构信息
Embryonic Stem Cell Group, Reliance Life Sciences Pvt. Ltd., Dhirubhai Ambani Life Sciences Center, R-282, TTC Industrial Area of MIDC, Thane-Belapur Road, Rabale, Navi Mumbai 400 701, India.
出版信息
Cell Prolif. 2006 Dec;39(6):585-98. doi: 10.1111/j.1365-2184.2006.00400.x.
Abstract
Embryonal carcinoma cells are pluripotent stem cells derived from teratocarcinomas and are considered to be the malignant counterparts of human embryonic stem cells. As there are few reliable experimental systems available to study the molecular mechanisms governing normal embryogenesis, well-characterized human embryonal carcinoma stem cell lines may provide a robust and simple model to study certain aspects of pluripotency and cellular differentiation. Here, we have analysed NTERA-2 cL.D1 cells at molecular and cellular levels during expansion and differentiation, via formation of cell aggregates similar to embryoid bodies in embryonic stem cells. Thus, human embryonal carcinoma cells may provide a valuable insight into cell fate determination, into the embryonic ectoderm, mesoderm and endoderm and their downstream derivatives.
摘要
胚胎癌细胞是源自畸胎癌的多能干细胞，被认为是人类胚胎干细胞的恶性对应物。由于可用于研究正常胚胎发生分子机制的可靠实验系统很少，特征明确的人类胚胎癌细胞系可能为研究多能性和细胞分化的某些方面提供一个强大而简单的模型。在这里，我们通过形成类似于胚胎干细胞中胚状体的细胞聚集体，在分子和细胞水平上分析了NTERA-2 cL.D1细胞在扩增和分化过程中的情况。因此，人类胚胎癌细胞可能为细胞命运决定、胚胎外胚层、中胚层和内胚层及其下游衍生物提供有价值的见解。
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