Acquadro Alberto, Portis Ezio, Moglia Andrea, Magurno Franco, Lanteri Sergio
Di.Va.P.R.A. Plant Genetics and Breeding, University of Turin, via L. da Vinci 44, I-10095 Grugliasco (Turin), Italy.
Genome. 2006 Sep;49(9):1149-59. doi: 10.1139/g06-074.
A high copy number of retrotransposon sequences are present and widely dispersed in plant genomes. Their activity generates a considerable degree of sequence polymorphism. Here, we report the cloning of CYRE-5, a long-terminal repeat carrying retrotransposon-like sequence in Cynara cardunculus L., and its exploitation to develop a DNA fingerprinting assay across 22 accessions, including both cultivated (globe artichoke and cultivated cardoon) and wild (wild cardoon) types. The effectiveness of the sequence-specific amplified polymorphism (S-SAP) platform is compared with that of amplified fragment length polymorphism (AFLP). A genetic linkage analysis, based on a hybrid population between 2 globe artichoke varietal types, resulted in the inclusion of 29 S-SAP loci in the core genetic map, confirming their dispersed distribution across the globe artichoke genome.
反转录转座子序列在植物基因组中具有高拷贝数且广泛分布。它们的活性产生了相当程度的序列多态性。在此，我们报道了CYRE-5的克隆，CYRE-5是一种在刺苞菜蓟中携带类反转录转座子序列的长末端重复序列，并利用它开发了一种针对22份材料的DNA指纹分析方法，这些材料包括栽培类型（球茎甘蓝和栽培刺菜蓟）和野生类型（野生刺菜蓟）。将序列特异性扩增多态性（S-SAP）平台的有效性与扩增片段长度多态性（AFLP）的有效性进行了比较。基于2种球茎甘蓝品种类型之间的杂交群体进行的遗传连锁分析，结果在核心遗传图谱中纳入了29个S-SAP位点，证实了它们在球茎甘蓝基因组中的分散分布。
