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正如X射线吸收光谱（XAS）所推断的那样，细胞色素f与质体蓝素结合后，其非典型的铁配位几何结构保持不变。

The atypical iron-coordination geometry of cytochrome f remains unchanged upon binding to plastocyanin, as inferred by XAS.

作者信息

Díaz-Moreno Irene, Díaz-Moreno Sofía, Subías Gloria, De la Rosa Miguel A, Díaz-Quintana Antonio

机构信息

Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla y Consejo Superior de Investigaciones Científicas, Américo Vespucio 49, 41092, Sevilla, Spain.

出版信息

Photosynth Res. 2006 Oct;90(1):23-8. doi: 10.1007/s11120-006-9102-8. Epub 2006 Nov 17.

DOI:10.1007/s11120-006-9102-8
PMID:17111237
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1769345/
Abstract

The transient complex between cytochrome f and plastocyanin from the cyanobacterium Nostoc sp. PCC 7119 has been analysed by X-ray Absorption Spectroscopy in solution, using both proteins in their oxidized and reduced states. Fe K-edge data mainly shows that the atypical metal coordination geometry of cytochrome f, in which the N-terminal amino acid acts as an axial ligand of the heme group, remains unaltered upon binding to its redox partner, plastocyanin. This fact suggests that cytochrome f provides a stable binding site for plastocyanin and minimizes the reorganization energy required in the transient complex formation, which could facilitate the electron transfer between the two redox partners.

摘要

通过溶液中的X射线吸收光谱法，使用处于氧化态和还原态的两种蛋白质，对来自蓝藻念珠藻属PCC 7119的细胞色素f和质体蓝素之间的瞬态复合物进行了分析。铁K边数据主要表明，细胞色素f的非典型金属配位几何结构（其中N端氨基酸作为血红素基团的轴向配体）在与它的氧化还原伙伴质体蓝素结合后保持不变。这一事实表明，细胞色素f为质体蓝素提供了一个稳定的结合位点，并使瞬态复合物形成所需的重组能最小化，这可能有助于两个氧化还原伙伴之间的电子转移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c6/1769345/1eda92406e8b/11120_2006_9102_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c6/1769345/3b59718278cf/11120_2006_9102_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c6/1769345/af9a36e22486/11120_2006_9102_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c6/1769345/1eda92406e8b/11120_2006_9102_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c6/1769345/3b59718278cf/11120_2006_9102_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c6/1769345/af9a36e22486/11120_2006_9102_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c6/1769345/1eda92406e8b/11120_2006_9102_Fig3_HTML.jpg

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1
The atypical iron-coordination geometry of cytochrome f remains unchanged upon binding to plastocyanin, as inferred by XAS.正如X射线吸收光谱（XAS）所推断的那样，细胞色素f与质体蓝素结合后，其非典型的铁配位几何结构保持不变。
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本文引用的文献

1
Cytochrome f: Structure, function and biosynthesis.细胞色素 f：结构、功能与生物合成。
Photosynth Res. 1992 Dec;34(3):359-74. doi: 10.1007/BF00029811.
2
Detecting transient protein-protein interactions by X-ray absorption spectroscopy: The cytochrome c6-photosystem I complex.
FEBS Lett. 2006 May 29;580(13):3023-8. doi: 10.1016/j.febslet.2006.04.045. Epub 2006 Apr 27.
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A comparative structural and functional analysis of cyanobacterial plastocyanin and cytochrome c (6) as alternative electron donors to Photosystem I.蓝藻质体蓝素和细胞色素c（6）作为光系统I替代电子供体的结构与功能比较分析
Photosynth Res. 2003;75(2):97-110. doi: 10.1023/A:1022841513592.
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Laser flash-induced kinetic analysis of cytochrome f oxidation by wild-type and mutant plastocyanin from the cyanobacterium Nostoc sp. PCC 7119.来自蓝细菌念珠藻属Nostoc sp. PCC 7119的野生型和突变体质体蓝素对细胞色素f氧化的激光闪光诱导动力学分析。
Biochemistry. 2005 Aug 30;44(34):11601-7. doi: 10.1021/bi050917g.
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Complexes of photosynthetic redox proteins studied by NMR.通过核磁共振研究光合氧化还原蛋白复合物。
Photosynth Res. 2004;81(3):277-87. doi: 10.1023/B:PRES.0000036880.67124.e7.
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The transient complex of poplar plastocyanin with cytochrome f: effects of ionic strength and pH.杨树质体蓝素与细胞色素f的瞬态复合物：离子强度和pH值的影响
Biochim Biophys Acta. 2005 Apr-May;1707(2-3):179-88. doi: 10.1016/j.bbabio.2004.12.002. Epub 2004 Dec 29.
7
Different modes of interaction in cyanobacterial complexes of plastocyanin and cytochrome f.质体蓝素和细胞色素f的蓝藻复合体中的不同相互作用模式。
Biochemistry. 2005 Mar 8;44(9):3176-83. doi: 10.1021/bi047855z.
8
Structure of the complex between plastocyanin and cytochrome f from the cyanobacterium Nostoc sp. PCC 7119 as determined by paramagnetic NMR. The balance between electrostatic and hydrophobic interactions within the transient complex determines the relative orientation of the two proteins.通过顺磁核磁共振确定的来自蓝细菌念珠藻属PCC 7119的质体蓝素和细胞色素f之间复合物的结构。瞬态复合物中静电相互作用和疏水相互作用之间的平衡决定了两种蛋白质的相对取向。
J Biol Chem. 2005 May 13;280(19):18908-15. doi: 10.1074/jbc.M413298200. Epub 2005 Feb 10.
9
Structure of the intermolecular complex between plastocyanin and cytochrome f from spinach.菠菜质体蓝素与细胞色素f之间分子间复合物的结构
J Biol Chem. 2005 May 13;280(19):18833-41. doi: 10.1074/jbc.M412760200. Epub 2005 Feb 3.
10
The linked conservation of structure and function in a family of high diversity: the monomeric cupredoxins.一个高度多样化家族中结构与功能的关联保守性：单体铜氧化还原蛋白。
Structure. 2004 Jun;12(6):917-25. doi: 10.1016/j.str.2004.03.029.