Huang Zhi-Ji, Huang Yi, Peng Bo
College of Environmental Sciences, Peking University, Beijing 100871, China.
Huan Jing Ke Xue. 2006 Aug;27(8):1654-8.
Ectomycorrhizal fungus has the ability to enhance the growth of higher plants in the contaminated area, especially ruined by heavy metals. And much attention was focused on how the fungus could enhance the resistance of higher plants. We focused on the resistance of ectomycorrhizal fungus in vitro to heavy metals. In the first experiment, the mycelium biomasses of two ectomycorhizal funguses growing in the Kottke media treated with different concentrations of Cu and Cd were measured after growth as well as the pH value of the medium. The results indicated that heavy metals could reduce the biomasses of the two funguses. Gomplhidius viscidus has higher tolerance to Cu but less Cd than that of Boletus edulis. With development of fungal mycila, the pH value of medium dropped significantly, and this effect might play an important role in enhancing its tolerance. In addition, the higher pH value change per biomass indicated that the fungus treated with heavy metals had the ability to adjust environment of pH more significantly. In the second experiment, the cation exchange capacity (CEC) of the cell walls of the fungus treated with heavy metals was measured according to Marschner's. The results indicated that with the increasing of the concentrations of Cu or Cd, the CEC of Gomphidius viscidus increased, but the CEC of Boletus edulis dropped.
外生菌根真菌具有促进污染区域(尤其是被重金属破坏的区域)高等植物生长的能力。并且,人们十分关注这种真菌如何增强高等植物的抗性。我们关注的是外生菌根真菌在体外对重金属的抗性。在第一个实验中,在不同浓度的铜(Cu)和镉(Cd)处理过的科特克培养基中生长的两种外生菌根真菌,在生长后测量其菌丝体生物量以及培养基的pH值。结果表明,重金属会降低这两种真菌的生物量。粘盖牛肝菌(Gomplhidius viscidus)对铜的耐受性高于美味牛肝菌(Boletus edulis),但对镉的耐受性低于美味牛肝菌。随着真菌菌丝的生长,培养基的pH值显著下降,这种效应可能在增强其耐受性方面发挥重要作用。此外,每生物量较高的pH值变化表明,经重金属处理的真菌具有更显著调节pH环境的能力。在第二个实验中,根据马斯纳(Marschner)的方法测量了经重金属处理的真菌细胞壁的阳离子交换容量(CEC)。结果表明,随着铜或镉浓度的增加,粘盖牛肝菌的阳离子交换容量增加,而美味牛肝菌的阳离子交换容量下降。