Chand N, Pillar J, Diamantis W, Sofia R D
Department of Pharmacology, Wallace Laboratories Division of Carter-Wallace, Inc., Cranbury, New Jersey 08512.
Res Commun Chem Pathol Pharmacol. 1991 Apr;72(1):121-4.
In this study the effect of azelastine on the activation (antigen-dependent, Ca(2+)-dependent, Stage I) and release (antigen-independent, Ca(2+)-dependent, Stage II) phases of allergen-induced histamine secretion in rabbit mixed leukocytes (basophils) was investigated. Azelastine (5 microM, 10-min) and diltiazem (5 microM: a Ca2+ antagonist, 10 min) inhibited ragweed extract-induced histamine secretion during the Stage II (release) phase. Theophylline (100 microM), a phosphodiesterase inhibitor, added immediately before antigen challenge, inhibited allergic histamine secretion during the Stage I (activation) phase. The data obtained in this study suggest that diltiazem and azelastine act on the Ca(2+)-dependent and antigen-independent phase (Stage II) of allergic histamine secretion in rabbit basophils.
在本研究中，研究了氮卓斯汀对兔混合白细胞（嗜碱性粒细胞）中变应原诱导的组胺分泌的激活阶段（抗原依赖性、Ca(2+)依赖性、第一阶段）和释放阶段（抗原非依赖性、Ca(2+)依赖性、第二阶段）的影响。氮卓斯汀（5微摩尔，10分钟）和地尔硫卓（5微摩尔：一种Ca2+拮抗剂，10分钟）在第二阶段（释放）抑制了豚草提取物诱导的组胺分泌。在抗原攻击前立即加入的磷酸二酯酶抑制剂茶碱（100微摩尔）在第一阶段（激活）抑制了过敏性组胺分泌。本研究获得的数据表明，地尔硫卓和氮卓斯汀作用于兔嗜碱性粒细胞中过敏性组胺分泌的Ca(2+)依赖性和抗原非依赖性阶段（第二阶段）。
