Deckhut A M, Tevethia M J, Haggerty S, Frisque R J, Tevethia S S
Department of Microbiology and Immunology, Pennsylvania State College of Medicine, Hershey 17033.
Virology. 1991 Jul;183(1):122-32. doi: 10.1016/0042-6822(91)90125-u.
Human papovavirus JC virus (JCV) and Simian virus 40 (SV40) tumor or T antigens demonstrate considerable sequence homology which is reflected by antibody cross-reactivity. This similarity raised the possibility that JCV and SV40 T antigen also might contain common cytotoxic T lymphocyte (CTL) recognition epitopes. In this study we identified and mapped such sites on the JCV T antigen. C57Bl/6 cell lines transformed by JCV/SV40 T antigen chimeras were generated and tested for susceptibility to lysis by five H-2b restricted SV40-specific CTL clones: Y-1, Y-2, Y-3, Y-4, and Y-5. These CTL clones recognize specific epitopes within amino acids 205-219 (site I), 220-233 (sites II and III), 369-511 (site IV), and 489-503 (site V) on SV40 T Ag, respectively. The results show that sites I, II, III, and IV (recognized by CTL clones Y-1, Y-2, Y-3, and Y-4, respectively) represent common epitopes on SV40 and JCV T antigens. CTL clone Y-5 failed to recognize JCV T antigen indicating that CTL can discriminate between the two antigenically related T antigens.
人乳头多瘤病毒JC病毒（JCV）和猴病毒40（SV40）的肿瘤或T抗原表现出相当程度的序列同源性，这通过抗体交叉反应得以体现。这种相似性增加了JCV和SV40 T抗原也可能含有共同的细胞毒性T淋巴细胞（CTL）识别表位的可能性。在本研究中，我们在JCV T抗原上鉴定并定位了这些位点。构建了由JCV/SV40 T抗原嵌合体转化的C57Bl/6细胞系，并检测其对五个H-2b限制性SV40特异性CTL克隆（Y-1、Y-2、Y-3、Y-4和Y-5）裂解的敏感性。这些CTL克隆分别识别SV40 T抗原上氨基酸205-219（位点I）、220-233（位点II和III）、369-511（位点IV）和489-503（位点V）内的特定表位。结果表明，位点I、II、III和IV（分别由CTL克隆Y-1、Y-2、Y-3和Y-4识别）代表SV40和JCV T抗原上的共同表位。CTL克隆Y-5未能识别JCV T抗原，表明CTL能够区分这两种抗原相关的T抗原。
