Tanaka Y, Tevethia S S
Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey 17033.
Intervirology. 1990;31(2-4):197-202. doi: 10.1159/000150154.
Five distinct antigenic sites recognized by cytotoxic T lymphocytes in H-2b mice have been identified on simian virus 40 (SV40) T antigen. Cocultivation of SV40-transformed cells with cytotoxic T lymphocyte (CTL) clones specific for epitopes I-IV results in the selection of variants which show a loss of either epitopes I, II, and III, II and III, or IV. We now report the isolation of a variant SV40-transformed cell line which has lost the expression of all five CTL epitopes on SV40 T antigen. These variant cells were no longer susceptible to lysis by CTL clones specific for all five epitopes. These variant cells would be used to relate alterations in SV40 T antigen structure with functional changes in the transformed cells.
在H - 2b小鼠中，细胞毒性T淋巴细胞所识别的猿猴病毒40（SV40）T抗原上的五个不同抗原位点已被确定。将SV40转化细胞与针对表位I - IV的细胞毒性T淋巴细胞（CTL）克隆共培养，会筛选出缺失表位I、II和III、II和III或IV的变体。我们现在报告分离出一种变体SV40转化细胞系，该细胞系在SV40 T抗原上失去了所有五个CTL表位的表达。这些变体细胞不再易被针对所有五个表位的CTL克隆裂解。这些变体细胞将用于关联SV40 T抗原结构的改变与转化细胞中的功能变化。
