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[空肠弯曲菌抗原在免疫酶分析中的特异性]

[The specificity of Campylobacter jejuni antigens in immunoenzyme analysis].

作者信息

Kapeliush E V, Nikolaeva T A, Safonova N V, Matveeva Z N, Khazenson L B

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1990 Nov(11):69-72.

PMID:1711263
Abstract

The surface antigen has been obtained from C. jejuni culture and its immunochemical analysis has been carried out by the methods of immunoelectrophoresis, double diffusion with homologous and heterologous rabbit sera. The protein profile of this antigen has been studied by means of vertical electrophoresis in polyacrylamide gel with sodium dodecyl sulfate, and the molecular weight of the protein components of the antigen has been determined. The presence of anti-Campylobacter antibodies has been revealed in a number of rabbit sera by enzyme immunoassay techniques. The results thus obtained indicate the possibility of developing the enzyme immunoassay for the determination of antibodies in the sera of patients with Campylobacter infection.

摘要

空肠弯曲菌培养物已获得表面抗原,并采用免疫电泳、与同源和异源兔血清进行双向扩散的方法对其进行了免疫化学分析。利用十二烷基硫酸钠聚丙烯酰胺凝胶垂直电泳法研究了该抗原的蛋白质谱,并测定了抗原蛋白质成分的分子量。通过酶免疫测定技术在一些兔血清中检测到了抗弯曲杆菌抗体。由此获得的结果表明,有可能开发一种酶免疫测定法来检测弯曲杆菌感染患者血清中的抗体。

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