玉米种子发育过程中直链淀粉积累的分析

Analysis of amylose accumulation during seed development in maize.

作者信息

Guo Shang-Jing, Li Jia-Rui, Qiao Wei-Hua, Zhang Xian-Sheng

机构信息

College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.

出版信息

Yi Chuan Xue Bao. 2006 Nov;33(11):1014-9. doi: 10.1016/S0379-4172(06)60137-6.

DOI:10.1016/S0379-4172(06)60137-6

PMID:17112973

Abstract

Starch, which includes amylose and amylopectin, is the most important component in maize (Zea mays L.) seeds. The accumulation of amylose in maize seeds was examined in this study. The percentage of amylose content gradually increased in seeds from day 10 to day 25 after pollination, which is consistent with the changes of GBSS activity. The transcripts of GBSSI were detected in both the endosperm and embryo of wild-type maize. However, its transcripts, GBSS activity, and amylose were not detected in either the endosperm or embryo of waxy maize. These results indicate that the accumulation of amylose is controlled by GBSSI expression in the seeds of maize.

摘要

淀粉包括直链淀粉和支链淀粉，是玉米（Zea mays L.）种子中最重要的成分。本研究检测了玉米种子中直链淀粉的积累情况。授粉后第10天到第25天，种子中直链淀粉含量的百分比逐渐增加，这与颗粒结合型淀粉合成酶（GBSS）活性的变化一致。在野生型玉米的胚乳和胚中均检测到GBSSI的转录本。然而，在糯玉米的胚乳和胚中均未检测到其转录本、GBSS活性和直链淀粉。这些结果表明，玉米种子中直链淀粉的积累受GBSSI表达的控制。