Thornton Brian R, Toczyski David P
Department of Biochemistry and Biophysics, Cancer Research Institute, University of California at San Francisco, San Francisco, California 94115, USA.
Genes Dev. 2006 Nov 15;20(22):3069-78. doi: 10.1101/gad.1478306.
Cell cycle transitions are often accompanied by the degradation of regulatory molecules. Targeting proteins to the proteasome for degradation is accomplished by the covalent addition of ubiquitin chains. The specificity of this pathway is largely dictated by a set of enzymes called ubiquitin ligases (or E3s). The anaphase-promoting complex (or APC) is a ubiquitin ligase that has a particularly prominent role in regulating cell cycle progression. To date, the APC is the most complicated member of the RING/cullin family of multisubunit E3s. It includes at least 13 core subunits and three related adaptors. A combination of biochemical, genetic, and structural approaches are now shedding light on the enzymology of the APC. This review will focus on these data, drawing parallels with related ubiquitin ligases.
细胞周期转换通常伴随着调节分子的降解。将蛋白质靶向蛋白酶体进行降解是通过泛素链的共价添加来实现的。该途径的特异性很大程度上由一组称为泛素连接酶（或E3）的酶决定。后期促进复合物（或APC）是一种泛素连接酶，在调节细胞周期进程中具有特别突出的作用。迄今为止，APC是多亚基E3的RING/ Culli n家族中最复杂的成员。它包括至少13个核心亚基和三个相关的衔接子。生物化学、遗传学和结构方法的结合现在正在揭示APC的酶学。本综述将重点关注这些数据，并与相关泛素连接酶进行比较。
