同时测定用对氨基苯甲酸防晒制剂处理的志愿者尿液中的对氨基苯甲酸及其代谢物。

Simultaneous determination of p-aminobenzoic acid and its metabolites in the urine of volunteers, treated with p-aminobenzoic acid sunscreen formulation.

作者信息

Wang Lai-Hao, Huang Wen-Shiuan, Tai Huo-Mu

机构信息

Department of Applied Chemistry, Chia Nan University of Pharmacy and Science, Tainan 71743, Taiwan.

出版信息

J Pharm Biomed Anal. 2007 Mar 12;43(4):1430-6. doi: 10.1016/j.jpba.2006.10.021. Epub 2006 Nov 21.

DOI:10.1016/j.jpba.2006.10.021

PMID:17118613

Abstract

p-Aminobenzoic acid (PABA) and its metabolites (p-aminohippuric acid, p-acetamidobenzoic acid, and p-acetamidohippuric acid) were detected using high-performance liquid chromatography with an electrochemical (carbon paste) detector (HPLC-ECD). For direct current (dc) mode, with the current at a constant potential, and measurements with suitable experimental parameters, a linear concentration from 0.125 to 1.80 microg/ml was found. The detection limit was approximately 2.0 ng/ml. A carbon paste coulometric detector was used to demonstrate that PABA and its metabolites are electrochemically oxidized in acidic media, and to determine, by analyzing human urine, the percutaneous absorption of PABA and its metabolites. Findings using HPLC-ECD and HPLC with an ultraviolet detector (HPLC-UV) were comparable.

摘要

采用高效液相色谱-电化学（碳糊）检测器（HPLC-ECD）检测对氨基苯甲酸（PABA）及其代谢产物（对氨基马尿酸、对乙酰氨基苯甲酸和对乙酰氨基马尿酸）。在直流（dc）模式下，保持电流在恒定电位，并采用合适的实验参数进行测量，发现浓度在0.125至1.80微克/毫升之间呈线性关系。检测限约为2.0纳克/毫升。使用碳糊库仑检测器证明PABA及其代谢产物在酸性介质中会发生电化学氧化，并通过分析人体尿液来测定PABA及其代谢产物的经皮吸收情况。使用HPLC-ECD和配备紫外检测器的HPLC（HPLC-UV）的检测结果具有可比性。