Schomburg Eike D, Steffens Heinz, Maznychenko Andrey V, Pilyavskii Alexander I, Hellström Fredrik, Kostyukov Alexander I, Maisky Vladimir A
Zentrum Physiologie und Pathophysiologie, Universität Göttingen, Humboldtallee 23, D-37073 Göttingen, Germany.
Eur J Pain. 2007 Jul;11(5):579-86. doi: 10.1016/j.ejpain.2006.09.004. Epub 2006 Nov 22.
The aim of this research was to study the changes of the motor reflex activity (monosynaptic reflex (MSR) of the flexor and extensor muscles) and Fos immunoreactivity in lumbo-sacral spinal cord after acute induced myositis of m. gastrocnemius-soleus (GS). The experiments were carried out on ischaemic decerebrated, spinalized in C1 cats. After infiltration of the GS muscle with carrageenan (2%) MSRs of flexors and extensors showed a significant increase in amplitude +127+/-24.5% and +155+/-28.5%, respectively, p<0.05. The exposed effect was initiated within 30 min and achieved a maximum 2.8h after the intramuscular injections of carrageenan. After analysis of dynamics of the MSRs, animals were perfused and c-fos expression in the spinal segments L6-S1 was evaluated. In comparison to sham-operated animals, the number of Fos-immunoreactive (Fos-ir) cells was noticeably increased in the lumbar cord of cats with carrageenan-induced myositis. The labeled cells were concentrated in the ipsilateral laminae I/II, neck of the dorsal horn (V/VI) and intermediate zone (VII), however, clear predominance of their concentration was found in the deep laminae. The effect of muscle inflammation was also expressed as a significant decline in the number of NADPH-d-reactive cells (p<0.05) in ipsilateral laminae I/II of L6/L7. The results show that the input from acutely inflamed muscles may induce an increase of the reflex responsiveness of flexors and extensors which is not mediated via the gamma-spindle-loop and which coincides with a significant increase in c-fos expression in the deep laminae of the lumbar spinal cord.
本研究的目的是研究急性诱导腓肠肌比目鱼肌（GS）肌炎后腰骶部脊髓运动反射活动（屈肌和伸肌的单突触反射（MSR））和Fos免疫反应性的变化。实验在C1节段脊髓横断、大脑缺血去大脑的猫身上进行。用角叉菜胶（2%）浸润GS肌肉后，屈肌和伸肌的MSR幅度分别显著增加+127±24.5%和+155±28.5%，p<0.05。在肌肉注射角叉菜胶后30分钟内开始出现上述效应，并在2.8小时后达到最大值。在分析MSR的动态变化后，对动物进行灌注，并评估L6-S1脊髓节段中的c-fos表达。与假手术动物相比，角叉菜胶诱导肌炎的猫的腰髓中Fos免疫反应性（Fos-ir）细胞数量明显增加。标记细胞集中在同侧的I/II层、背角颈部（V/VI）和中间带（VII），然而，在深层中发现它们的浓度明显占优势。肌肉炎症的影响还表现为L6/L7同侧I/II层中NADPH-d反应性细胞数量显著下降（p<0.05）。结果表明，急性炎症肌肉的传入可能会诱导屈肌和伸肌反射反应性增加，这不是通过γ-梭内肌环路介导的，并且与腰髓深层中c-fos表达的显著增加相一致。
