氯霉素和放线菌酮对脑胆碱能突触体蛋白质合成抑制作用的评估。

Estimation of the chloramphenicol and cycloheximide inhibition of protein synthesis in brain cholinergic synaptosomes.

作者信息

Corbaton V, Muiño M T, Fernández-Silva P, López-Pérez M J, Montoya J

机构信息

Departamento de Bioqumica, Biología Molecular y Celular, Facultad de Veterinaria, Zaragoza, Spain.

出版信息

Brain Res. 1991 Mar 15;543(2):351-3. doi: 10.1016/0006-8993(91)90049-2.

DOI:10.1016/0006-8993(91)90049-2

PMID:1711913

Abstract

Cholinergic synaptosomes have been prepared from sheep brain cortex by means of an immunoaffinity method using a specific anti-(Chol I) antiserum. The [14C]leucine incorporation into proteins of this preparation shows a low cycloheximide and a high chloramphenicol sensitivity. This fact suggests that the mitochondrial protein synthesis system is the only one present in this fraction.

摘要

已通过使用特异性抗（Chol I）抗血清的免疫亲和方法从绵羊脑皮质制备了胆碱能突触体。该制剂中蛋白质的[14C]亮氨酸掺入显示出低放线菌酮敏感性和高氯霉素敏感性。这一事实表明线粒体蛋白质合成系统是该部分中唯一存在的系统。