Tolivia D, Tolivia J
Dept. Morfología y Biología Celular, Universidad de Oviedo, Spain.
J Neurosci Methods. 1991 Feb;36(2-3):139-43. doi: 10.1016/0165-0270(91)90039-3.
A simple and rapid method for the impregnation of neuronal bodies applicable to methacrylate embedded sections is described in the present paper. Sections of 10-12 microns in thickness were attached to slides, placed in mordant for 1 min, rinsed in distilled water and impregnated in ammoniacal silver solution for 1 min. They were then rinsed in absolute ethanol for 30 s and developed in 50% formalin. Sections were toned in 0.25% gold chloride, reduced in 10% oxalic acid and fixed in 5% sodium thiosulfate. After washing, the sections were dehydrated through 90% and absolute ethanol, cleared in eucalyptol, and mounted in the usual way. When this method is used most of the neuronal somata and proximal dendritic trees are impregnated. Frequently some glial cell are also weakly impregnated but their density does not obscure the neurons.
本文描述了一种适用于甲基丙烯酸酯包埋切片的简单快速的神经元胞体浸染方法。将厚度为10 - 12微米的切片贴在载玻片上，置于媒染剂中1分钟，用蒸馏水冲洗，再浸入氨性银溶液中1分钟。然后在无水乙醇中冲洗30秒，在50%福尔马林中显影。切片用0.25%氯化金调色，用10%草酸还原，并用5%硫代硫酸钠固定。洗涤后，切片依次通过90%乙醇和无水乙醇脱水，用桉叶油透明，然后按常规方法封片。使用该方法时，大多数神经元胞体和近端树突都能被浸染。通常一些胶质细胞也会被轻微浸染，但它们的密度不会掩盖神经元。
