克隆并表达的一氧化氮合酶在结构上类似于细胞色素P-450还原酶。
Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase.
作者信息
Bredt D S, Hwang P M, Glatt C E, Lowenstein C, Reed R R, Snyder S H
机构信息
Department of Neuroscience, Johns Hopkins Medical Institutions, Baltimore, Maryland 21205.
出版信息
Nature. 1991 Jun 27;351(6329):714-8. doi: 10.1038/351714a0.
Nitric oxide is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. Cloning of a complementary DNA for brain nitric oxide synthase reveals recognition sites for NADPH, FAD, flavin mononucleotide and calmodulin as well as phosphorylation sites, indicating that the synthase is regulated by many different factors. The only known mammalian enzyme with close homology is cytochrome P-450 reductase.
一氧化氮是一种信使分子，介导血管中内皮衍生舒张因子的作用以及巨噬细胞的细胞毒性作用，并在大脑的神经元通讯中发挥作用。脑一氧化氮合酶互补DNA的克隆揭示了NADPH、FAD、黄素单核苷酸和钙调蛋白的识别位点以及磷酸化位点，表明该合酶受多种不同因素调节。唯一已知的具有密切同源性的哺乳动物酶是细胞色素P-450还原酶。
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