Salas Joaquín J, Martínez-Force Enrique, Garcés Rafael
Instituto de la Grasa (CSIC), Av. Padre García Tejero 4, Sevilla 41012, Spain.
Lipids. 2006 Aug;41(8):805-11. doi: 10.1007/s11745-006-5034-5.
Phospholipids are essential components of plant cell membranes whose acyl composition appears to be influenced by oil composition in the sunflower. In the current study, we have determined the diacylglycerol profile of the main phospholipids using phospholipase C degradation and separation of the diacylglycerols by HPLC and GLC. The main polar lipid molecular species were defined in different classes of sunflower kernel: PC, PE, and PI. The proportions of each were determined at different stages of development in order to define the point at which the mutations carried by each sunflower line affected the phospholipid composition of the seeds. The results indicated that modifications to intraplastidial de novo FA synthesis affected the seed phospholipid profile during the whole period of the seed formation, including accumulation and maturation, whereas the influence of mutations in the endoplasmic reticulum desaturases were more readily detected at later stages of development. These results are discussed in terms of the pathways involved in glycerolipid synthesis and phospholipid conversion in sunflower seeds.
磷脂是植物细胞膜的重要组成部分，其酰基组成似乎受向日葵油成分的影响。在本研究中，我们利用磷脂酶C降解法，并通过高效液相色谱法（HPLC）和气相色谱法（GLC）分离二酰基甘油，确定了主要磷脂的二酰基甘油谱。在不同类别的向日葵籽仁中定义了主要的极性脂质分子种类：磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）和磷脂酰肌醇（PI）。为了确定每个向日葵品系携带的突变影响种子磷脂组成的时间点，测定了它们在不同发育阶段的比例。结果表明，质体内从头脂肪酸合成的改变在种子形成的整个时期，包括积累和成熟阶段，都会影响种子的磷脂谱，而内质网去饱和酶突变的影响在发育后期更容易检测到。本文根据向日葵种子中甘油脂合成和磷脂转化所涉及的途径对这些结果进行了讨论。
