Wang Clifford L, Wang Bruce B, Bartha Gábor, Li Lauri, Channa Namitha, Klinger Mark, Killeen Nigel, Wabl Matthias
Department of Microbiology and Immunology, University of California-San Francisco, San Francisco, CA 94143, USA.
Proc Natl Acad Sci U S A. 2006 Dec 5;103(49):18680-4. doi: 10.1073/pnas.0609030103. Epub 2006 Nov 22.
Retroviruses can cause tumors when they integrate near a protooncogene or tumor suppressor gene of the host. We infected >2,500 mice with the SL3-3 murine leukemia virus; in 22 resulting tumors, we found provirus integrations nearby or within the gene that contains the mir-17-92 microRNA (miRNA) cistron. Using quantitative real-time PCR, we showed that expression of miRNA was increased in these tumors, indicating that retroviral infection can induce expression of oncogenic miRNAs. Our results demonstrate that retroviral mutagenesis can be a potent tool for miRNA discovery.
逆转录病毒整合到宿主原癌基因或肿瘤抑制基因附近时可引发肿瘤。我们用SL3 - 3小鼠白血病病毒感染了2500多只小鼠；在产生的22个肿瘤中，我们发现前病毒整合到了包含mir - 17 - 92微小RNA（miRNA）顺反子的基因附近或基因内部。通过定量实时PCR，我们发现这些肿瘤中miRNA的表达增加，这表明逆转录病毒感染可诱导致癌miRNA的表达。我们的结果表明，逆转录病毒诱变可能是发现miRNA的有效工具。
