前病毒整合激活致癌性微小RNA顺反子。
Activation of an oncogenic microRNA cistron by provirus integration.
作者信息
Wang Clifford L, Wang Bruce B, Bartha Gábor, Li Lauri, Channa Namitha, Klinger Mark, Killeen Nigel, Wabl Matthias
机构信息
Department of Microbiology and Immunology, University of California-San Francisco, San Francisco, CA 94143, USA.
出版信息
Proc Natl Acad Sci U S A. 2006 Dec 5;103(49):18680-4. doi: 10.1073/pnas.0609030103. Epub 2006 Nov 22.
Abstract
Retroviruses can cause tumors when they integrate near a protooncogene or tumor suppressor gene of the host. We infected >2,500 mice with the SL3-3 murine leukemia virus; in 22 resulting tumors, we found provirus integrations nearby or within the gene that contains the mir-17-92 microRNA (miRNA) cistron. Using quantitative real-time PCR, we showed that expression of miRNA was increased in these tumors, indicating that retroviral infection can induce expression of oncogenic miRNAs. Our results demonstrate that retroviral mutagenesis can be a potent tool for miRNA discovery.
摘要
逆转录病毒整合到宿主原癌基因或肿瘤抑制基因附近时可引发肿瘤。我们用SL3 - 3小鼠白血病病毒感染了2500多只小鼠；在产生的22个肿瘤中，我们发现前病毒整合到了包含mir - 17 - 92微小RNA（miRNA）顺反子的基因附近或基因内部。通过定量实时PCR，我们发现这些肿瘤中miRNA的表达增加，这表明逆转录病毒感染可诱导致癌miRNA的表达。我们的结果表明，逆转录病毒诱变可能是发现miRNA的有效工具。
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本文引用的文献
1
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EMBO J. 2006 Jul 26;25(14):3422-31. doi: 10.1038/sj.emboj.7601215. Epub 2006 Jul 6.
2
Clarifications on miRNA and cancer.关于微小RNA与癌症的阐释
Science. 2006 Jan 6;311(5757):36-7. doi: 10.1126/science.311.5757.36d.
3
miRBase: microRNA sequences, targets and gene nomenclature.miRBase：微小RNA序列、靶标及基因命名法。
Nucleic Acids Res. 2006 Jan 1;34(Database issue):D140-4. doi: 10.1093/nar/gkj112.
4
Real-time quantification of microRNAs by stem-loop RT-PCR.通过茎环逆转录聚合酶链反应对微小核糖核酸进行实时定量分析
Nucleic Acids Res. 2005 Nov 27;33(20):e179. doi: 10.1093/nar/gni178.
5
Retroviral insertional mutagenesis: past, present and future.逆转录病毒插入诱变：过去、现在与未来。
Oncogene. 2005 Nov 21;24(52):7656-72. doi: 10.1038/sj.onc.1209043.
6
A polycistronic microRNA cluster, miR-17-92, is overexpressed in human lung cancers and enhances cell proliferation.一个多顺反子微小RNA簇miR-17-92在人类肺癌中过表达，并增强细胞增殖。
Cancer Res. 2005 Nov 1;65(21):9628-32. doi: 10.1158/0008-5472.CAN-05-2352.
7
microPrimer: the biogenesis and function of microRNA.微引物：微小RNA的生物合成与功能
Development. 2005 Nov;132(21):4645-52. doi: 10.1242/dev.02070.
8
MicroRNA gene expression deregulation in human breast cancer.人类乳腺癌中MicroRNA基因表达失调
Cancer Res. 2005 Aug 15;65(16):7065-70. doi: 10.1158/0008-5472.CAN-05-1783.
9
A tumor-suppressor function for NFATc3 in T-cell lymphomagenesis by murine leukemia virus.NFATc3在鼠白血病病毒诱导的T细胞淋巴瘤发生中的肿瘤抑制功能。
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10
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