Simmaco M, Barra D, Chiarini F, Noviello L, Melchiorri P, Kreil G, Richter K
Department of Biochemical Sciences, Università di Roma La Sapienza, Italy.
Eur J Biochem. 1991 Jul 1;199(1):217-22. doi: 10.1111/j.1432-1033.1991.tb16112.x.
A peptide fraction was isolated from the skin of Bombina variegata that showed antimicrobial activity. This fraction contained several molecular species, all of them consisting of 27 amino acid residues, with a constant C-terminal region (from residues 14-27), including an amidated carboxyl end and a variable N-terminal segment. These peptides are related but not identical to bombinin [Csordas, A. & Michl, H. (1970) Monatsh. Chem. 101, 182-189]. By using synthetic oligonucleotides corresponding to the C-terminal region of the peptides, a cDNA library from the skin of B. variegata was screened and several positive clones coding for the corresponding peptide precursors were isolated and sequenced. Each clone contained the genetic information for a different bombinin-like peptide. The antimicrobial activity towards different bacterial species of a synthetic peptide corresponding to one of the variants deduced from cDNA sequences was tested. This peptide was found to be mainly active against different isolates of Staphylococci and Escherichia coli.
从东方铃蟾皮肤中分离出一种具有抗菌活性的肽组分。该组分包含几种分子种类，它们均由27个氨基酸残基组成，具有恒定的C末端区域（从第14至27位残基），包括一个酰胺化的羧基末端和一个可变的N末端片段。这些肽与铃蟾肽相关但不相同[Csordas, A. & Michl, H. (1970) Monatsh. Chem. 101, 182 - 189]。通过使用与这些肽的C末端区域相对应的合成寡核苷酸，筛选了来自东方铃蟾皮肤的cDNA文库，并分离和测序了几个编码相应肽前体的阳性克隆。每个克隆都包含一种不同的铃蟾肽样肽的遗传信息。测试了与从cDNA序列推导的变体之一相对应的合成肽对不同细菌物种的抗菌活性。发现该肽主要对葡萄球菌和大肠杆菌的不同分离株具有活性。
