邦克酸和苍术苷抑制大鼠心脏线粒体膜上的氯离子通道。
Bongkrekic acid and atractyloside inhibits chloride channels from mitochondrial membranes of rat heart.
作者信息
Malekova Lubica, Kominkova Viera, Ferko Miroslav, Stefanik Peter, Krizanova Olga, Ziegelhöffer Attila, Szewczyk Adam, Ondrias Karol
机构信息
Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlarska 5, 833 34 Bratislava, Slovak Republic.
出版信息
Biochim Biophys Acta. 2007 Jan;1767(1):31-44. doi: 10.1016/j.bbabio.2006.10.004. Epub 2006 Oct 14.
The aim of this work was to characterize the effect of bongkrekic acid (BKA), atractyloside (ATR) and carboxyatractyloside (CAT) on single channel properties of chloride channels from mitochondria. Mitochondrial membranes isolated from a rat heart muscle were incorporated into a bilayer lipid membrane (BLM) and single chloride channel currents were measured in 250/50 mM KCl cis/trans solutions. BKA (1-100 microM), ATR and CAT (5-100 microM) inhibited the chloride channels in dose-dependent manner. The inhibitory effect of the BKA, ATR and CAT was pronounced from the trans side of a BLM and it increased with time and at negative voltages (trans-cis). These compounds did not influence the single channel amplitude, but decreased open dwell time of channels. The inhibitory effect of BKA, ATR and CAT on the mitochondrial chloride channel may help to explain some of their cellular and/or subcellular effects.
这项工作的目的是表征膨压素（BKA）、苍术苷（ATR）和羧基苍术苷（CAT）对线粒体氯离子通道单通道特性的影响。从大鼠心肌分离的线粒体膜被整合到双层脂质膜（BLM）中，并在250/50 mM KCl顺式/反式溶液中测量单氯离子通道电流。BKA（1 - 100 microM）、ATR和CAT（5 - 100 microM）以剂量依赖性方式抑制氯离子通道。BKA、ATR和CAT的抑制作用从BLM的反式侧明显，并且随着时间和负电压（反式 - 顺式）增加。这些化合物不影响单通道幅度，但减少通道的开放驻留时间。BKA、ATR和CAT对线粒体氯离子通道的抑制作用可能有助于解释它们的一些细胞和/或亚细胞效应。
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