Xia Cai-Fen, Lv Long, Chen Xin-You, Fu Bo-Qiao, Lei Ke-Lin, Qin Cai-Qin, Liu Yi
School of Chemistry and Materials Science, Hubei Engineering University, Xiaogan, 432000, People's Republic of China.
J Membr Biol. 2015 Apr;248(2):319-26. doi: 10.1007/s00232-015-9773-1. Epub 2015 Feb 4.
The production capacity and yield of neodymium (Nd) in China have ranked the first in the world. Because of its unique biophysical and biochemical properties, Nd compounds have entered into the agricultural environment greatly to promote plant growth. Mitochondria play a crucial role in respiration and metabolism during the growth of plants. However, little is known about the mechanism by which Nd act at the mitochondrial level in plant cells. In this study, rice mitochondrial swelling, collapsed transmembrane potential and decreased membrane fluidity were examined to be important factors for mitochondria permeability transition pore (mPTP) opening induced by Nd(III). The protection of cyclosporin A (CsA) and dithiothreitol (DTT) could confirm that Nd(III) could trigger mPTP opening. Additionally, mitochondrial membrane breakdown observed by TEM and the release of cytochrome c (Cyt c) could also elucidate the mPTP opening from another point of view. At last, the study showed that Nd(III) could restrain the mitochondrial membrane lipid peroxide, so it might interact with anionic lipid too. This detection will be conductive to the safe application of Nd compounds in agriculture and food industry.
中国钕（Nd）的生产能力和产量位居世界第一。由于其独特的生物物理和生化特性，钕化合物大量进入农业环境以促进植物生长。线粒体在植物生长过程中的呼吸和代谢中起着至关重要的作用。然而，关于钕在植物细胞线粒体水平上发挥作用的机制却知之甚少。在本研究中，水稻线粒体肿胀、跨膜电位崩溃和膜流动性降低被检测为钕（III）诱导线粒体通透性转换孔（mPTP）开放的重要因素。环孢菌素A（CsA）和二硫苏糖醇（DTT）的保护作用可以证实钕（III）能够触发mPTP开放。此外，通过透射电子显微镜（TEM）观察到的线粒体膜破裂以及细胞色素c（Cyt c）的释放也能从另一个角度阐明mPTP开放。最后，研究表明钕（III）可以抑制线粒体膜脂质过氧化，因此它可能也与阴离子脂质相互作用。该检测将有助于钕化合物在农业和食品工业中的安全应用。
