Ishihara A, Taguchi S, Araki H, Nishihira Y
College of Liberal Arts and Sciences, Kyoto University, Japan.
Neurosci Lett. 1991 Apr 1;124(2):141-3. doi: 10.1016/0304-3940(91)90079-9.
Extensor digitorum longus motoneurons in the rat spinal cord were identified by retrograde labeling with two fluorescent tracers, Fast blue (FB) and Nuclear yellow (NY). Labeled motoneurons had a blue fluorescent cytoplasm at 360 nm excitation wavelength with FB, and a golden-yellow fluorescent nucleus with NY on the cryostat section. Labeled motoneurons were further examined for succinate dehydrogenase activity on the same section used for identification of the motoneurons. This study demonstrates that fluorescent dyes are useful for neuroanatomical studies by the retrograde axonal transport method, and that quantitative analysis of metabolic activity in labeled motoneurons is also possible.
通过用两种荧光示踪剂，即快蓝（FB）和核黄（NY）进行逆行标记，鉴定了大鼠脊髓中的趾长伸肌运动神经元。在低温切片上，标记的运动神经元在360nm激发波长下用FB呈现蓝色荧光细胞质，用NY呈现金黄色荧光细胞核。在用于鉴定运动神经元的同一切片上，进一步检测标记运动神经元的琥珀酸脱氢酶活性。本研究表明，荧光染料通过逆行轴突运输方法对神经解剖学研究有用，并且对标记运动神经元的代谢活性进行定量分析也是可能的。
