一种用于检测胃活检组织中幽门螺杆菌感染的内部快速尿素酶试验的敏感性和特异性。

Sensitivity and specificity of an in-house rapid urease test for detecting Helicobacter pylori infection on gastric biopsy.

作者信息

Chomvarin Chariya, Chantarasuk Yingrit, Mairiang Pisaln, Kularbkaew Churairat, Sangchan Apichat, Chanlertrith Kitti, Namwat Wises

机构信息

Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.

出版信息

Southeast Asian J Trop Med Public Health. 2006 Mar;37(2):312-9.

PMID:17124992

Abstract

We developed an in-house rapid urease test (iRUT) and evaluated the efficacy and the agreement of the iRUT and the cRUT compared with culture and histology for the detection of H. pylori infection. Five iRUT media were tested with H. pylori isolates and other bacteria. The most suitable iRUT medium was further evaluated for detection of H. pylori infection. Gastric biopsies from 120 patients were diagnosed by culture, iRUT, cRUT and histology. The results of the iRUT and cRUT were read at 30 minutes, 1 hour and up to 24 hours. A true positive result was either the culture or both the RUT (cRUT or iRUT) and the histological examination being positive. The sensitivity and specificity of the iRUT result at 30 minutes, 1 hour and up to 24 hours were 77.1% and 100%, 77.6% and 100%, and 94.1% and 94.2%, respectively. Values for the same parameters of cRUT were 87.5% and 100%, 89.8% and 100%, and 100% and 94.2%, respectively. The agreement between the iRUT and cRUT was very good (kappa values > or = 0.82). Our results indicate that the iRUT is a-sensitive, specific and cost effective test. It can be appropriately applied for detecting H. pylori infection in gastric biopsy specimens.

摘要

我们研发了一种内部快速尿素酶试验（iRUT），并评估了iRUT和市售快速尿素酶试验（cRUT）与培养及组织学检查相比在检测幽门螺杆菌感染方面的效能和一致性。用幽门螺杆菌分离株及其他细菌对五种iRUT培养基进行了测试。对最适宜的iRUT培养基进一步评估其对幽门螺杆菌感染的检测能力。对120例患者的胃活检标本进行培养、iRUT、cRUT及组织学检查。iRUT和cRUT的结果分别在30分钟、1小时及长达24小时时读取。真阳性结果为培养阳性或RUT（cRUT或iRUT）及组织学检查均为阳性。iRUT在30分钟、1小时及长达24小时时结果的敏感性和特异性分别为77.1%和100%、77.6%和100%、94.1%和94.2%。cRUT相同参数的值分别为87.5%和100%、89.8%和100%、100%和94.2%。iRUT与cRUT之间的一致性非常好（kappa值≥0.82）。我们的结果表明，iRUT是一种敏感、特异且经济有效的检测方法。它可适用于检测胃活检标本中的幽门螺杆菌感染。