Medynski Dan, Tuan Michael, Liu Wayne, Wu Shili, Lin Xinli
Proteomtech Inc., 3505 Cadillac Ave., Building F7, Costa Mesa, CA 92626, USA.
Protein Expr Purif. 2007 Apr;52(2):395-402. doi: 10.1016/j.pep.2006.10.012. Epub 2006 Oct 26.
Two des-kringle derivatives of human plasminogen, microplasminogen and miniplasminogen, have been expressed at high levels as inclusion bodies in Escherichia coli using a T7 expression system. In each case, the isolated inclusion bodies were refolded and purified. A final yield of approximately 10% of total refolded protein was observed in each case. Both refolded molecules were successfully activated to their functional forms, microplasmin and miniplasmin, by the plasminogen activator urokinase. The kinetic properties of the refolded microplasmin and miniplasmin were comparable to full length, native plasmin.
人纤溶酶原的两种去kringle结构域衍生物，微纤溶酶原和迷你纤溶酶原，已使用T7表达系统在大肠杆菌中作为包涵体高水平表达。在每种情况下，分离得到的包涵体都进行了复性和纯化。每种情况下都观察到最终产量约为复性总蛋白的10%。两种复性分子都被纤溶酶原激活剂尿激酶成功激活为其功能形式，即微纤溶酶和迷你纤溶酶。复性后的微纤溶酶和迷你纤溶酶的动力学特性与全长天然纤溶酶相当。
