Furuto D K, Gay R E, Stewart T E, Miller E J, Gay S
Research Center in Oral Biology, University of Alabama, Birmingham 35294.
Matrix. 1991 Apr;11(2):144-9. doi: 10.1016/s0934-8832(11)80218-0.
Monoclonal antibodies produced against pepsin-solubilized newborn rat skin type V collagen [alpha 1(V)]2 alpha 2(V), and chondrosarcoma type XI collagen [alpha 1(XI) alpha 2(XI) alpha 3(XI)] are used to localize the collagens in sections of the chondrosarcoma as well as the normal rat knee joint by indirect immunofluorescence. Immunostaining for type V collagen shows strong cellular staining of chondrocytes; while the interstitial matrix as well as the lacunae are not stained. In contrast, antitype XI stains not only chondrocytes, but the extracellular compartments as well. In ELISA, rat anti-type XI collagen reacts with its native antigen, but does not cross-react with native types I, II, III, or V collagen from rat. The distinct locations of type V and XI collagens in cartilaginous tissue suggest varied functional roles for these constituents in the tissue.
针对胃蛋白酶溶解的新生大鼠皮肤V型胶原蛋白[α1(V)]2α2(V)和软骨肉瘤XI型胶原蛋白[α1(XI)α2(XI)α3(XI)]产生的单克隆抗体，通过间接免疫荧光法用于在软骨肉瘤切片以及正常大鼠膝关节中定位这些胶原蛋白。V型胶原蛋白的免疫染色显示软骨细胞有强烈的细胞染色；而间质基质以及腔隙未被染色。相比之下，XI型抗体不仅对软骨细胞染色，而且对细胞外区域也进行染色。在酶联免疫吸附测定（ELISA）中，大鼠抗XI型胶原蛋白与天然抗原发生反应，但不与大鼠的天然I型、II型、III型或V型胶原蛋白发生交叉反应。V型和XI型胶原蛋白在软骨组织中的不同定位表明这些成分在组织中具有不同的功能作用。
