Liu Shu-ying, Fan Hao-jun, Qian Gui-sheng, Zhang Jian-peng
Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2006 Oct;29(10):674-8.
To explore the suppressive effects of perfluorocarbon (PFC, C(8)F(18)) injected intraperitoneally on neutrophilic infiltration in lipopolysaccharide (LPS)-induced lung injury in rats.
A hundred and eight Wistar rats were randomly divided into 4 groups, and with 9 rats in each of the 3 time points respectively: Normal control group (NC group), PFC control group (PFC group), LPS-induced acute lung injury group (LPS group) and PFC pre-treatment group (PFC + LPS group). The animals were injected intraperitoneally with PFC at dose of 15 ml/kg in PFC group and PFC + LPS group 48 hours before. LPS at dose of 7 mg/kg injected via penile vein induced lung injury at LPS group and PFC + LPS group and the animals were respectively killed by exsanguination at 2, 4, 6 hour points. The white blood cells and neutrophils were counted in bronchoalveolar lavage fluid (BALF) and venous blood, and the neutrophils were counted in lung tissue under microscope. The expression of E-selectin and intercellular adhesion molecule-1 (ICAM-1) were immunohistochemically detected in lung tissue as integrated optic density (IOD) measured with Image Pro Plus 5.1 software.
(1) The counts of white blood cells of BALF [(1.98 +/- 0.21), (2.98 +/- 0.43), (3.95 +/- 0.29) x 10(6)/L at three times points respectively] and PMN percentages (0.170 +/- 0.069, 0.250 +/- 0.046, 0.351 +/- 0.054 at times points respectively) in LPS group significantly increased as compared with those in NC group [(1.27 +/- 0.20), (1.27 +/- 0.18), (1.26 +/- 0.11) x 10(6)/L and 0.041 +/- 0.008, 0.041 +/- 0.007, 0.041 +/- 0.007 at three times points respectively, t = 5.680 - 18.924, all P < 0.01]. The counts of white blood cells and PMN percentages of BALF in PFC + LPS group [(1.45 +/- 0.39), (2.67 +/- 0.44), (3.29 +/- 0.45) x 10(6)/L and 0.065 +/- 0.024, 0.102 +/- 0.033, 0.174 +/- 0.049 at times points respectively] significantly reduced as compared with those of LPS group (t = -4.224 - 12.033, P < 0.01). (2) On the contrary, the counts of white blood cells of venous blood in LPS group [(5.26 +/- 0.85), (4.38 +/- 0.39) x 10(9)/L at 4, 6 h] were significantly lower than NC group [(6.29 +/- 0.55), (6.28 +/- 0.60) x 10(9)/L, t = -3.088 and -7.946, P < 0.01], whereas the PFC pre-treatment significantly increased the counts. (3) In lung tissue, the counts of PMN per field (7.56 +/- 1.81, 18.76 +/- 3.51 and 33.99 +/- 5.68), the expressions of E-selectin (IOD: 208 +/- 78, 283 +/- 67 and 625 +/- 85) and ICAM-1 (IOD: 208 +/- 78, 283 +/- 67 and 625 +/- 85) in PFC + LPS group were significantly decreased compared with LPS group (10.78 +/- 0.92, 31.55 +/- 3.00 and 54.14 +/- 5.49; 1,086 +/- 256, 1,606 +/- 408 and 3,409 +/- 1,751; 299 +/- 97, 378 +/- 67 and 817 +/- 149, respectively, t = -2.400 - 11.480, P < 0.01) at three time points.
PFC pre-injected intraperitoneally significantly suppressed the neutrophilic infiltration in LPS-induced lung injury and reduced the expressions of E-selectin and ICAM-1 as the possible molecular mechanism of its effects.
探讨腹腔注射全氟碳化合物（PFC，C(8)F(18)）对脂多糖（LPS）诱导的大鼠肺损伤中性粒细胞浸润的抑制作用。
108只Wistar大鼠随机分为4组，在3个时间点各9只：正常对照组（NC组）、PFC对照组（PFC组）、LPS诱导的急性肺损伤组（LPS组）和PFC预处理组（PFC + LPS组）。PFC组和PFC + LPS组在48小时前腹腔注射剂量为15 ml/kg的PFC。LPS组和PFC + LPS组经阴茎静脉注射剂量为7 mg/kg的LPS诱导肺损伤，并分别在2、4、6小时处死动物取血。对支气管肺泡灌洗液（BALF）和静脉血中的白细胞和中性粒细胞进行计数，并在显微镜下对肺组织中的中性粒细胞进行计数。采用免疫组织化学方法检测肺组织中E-选择素和细胞间黏附分子-1（ICAM-1）的表达，并使用Image Pro Plus 5.1软件测量积分光密度（IOD）。
（1）LPS组BALF中白细胞计数[三个时间点分别为(1.98 ± 0.21)、(2.98 ± 0.43)、(3.95 ± 0.29)×10(6)/L]和PMN百分比[三个时间点分别为0.170 ± 0.069、0.250 ± 0.046、0.351 ± 0.054]与NC组[三个时间点分别为(1.27 ± 0.20)、(1.27 ± 0.18)、(1.26 ± 0.11)×10(6)/L和0.041 ± 0.008、0.041 ± 0.007、0.041 ± 0.007]相比显著增加（t = 5.680 - 18.924，均P < 0.01）。PFC + LPS组BALF中白细胞计数和PMN百分比[三个时间点分别为(1.45 ± 0.39)、(2.67 ± 0.44)、(3.29 ± 0.45)×10(6)/L和0.065 ± 0.024、0.102 ± 0.033、0.174 ± 0.049]与LPS组相比显著降低（t = -4.224 - 12.033，P < 0.01）。（2）相反，LPS组静脉血白细胞计数[4、6小时时分别为(5.26 ± 0.85)、(4.38 ± 0.39)×10(9)/L]显著低于NC组[(6.29 ± 0.55)、(6.28 ± 0.60)×10(9)/L，t = -3.088和-7.946，P < 0.01]，而PFC预处理显著增加了白细胞计数。（3）在肺组织中，PFC + LPS组三个时间点每视野PMN计数（7.56 ± 1.81、18.76 ± 3.51和33.99 ± 5.68）、E-选择素表达（IOD：208 ± 78、283 ± 67和625 ± 85）和ICAM-1表达（IOD：208 ± 78、283 ± 67和625 ± 85）与LPS组（分别为10.78 ± 0.92、31.55 ± 3.00和
