Buchan A M, Kwok Y N, Pederson R A
Department of Physiology, University of British Columbia, Vancouver, Canada.
Regul Pept. 1991 Jun 11;34(1):1-12. doi: 10.1016/0167-0115(91)90219-7.
Injections of the retrograde tracers into the posterior surface of the stomach at the greater curvature resulted in labelling of the right half of the dorsal motor nucleus of the vagus. Whereas injections into the anterior and posterior surfaces of the corpus resulted in bilateral labelling in the medulla. Immunocytochemical staining of the labelled sections using antisera to substance P was confined to a dense network of fibers within the dorsal motor nucleus of the vagus and the nucleus tractus solitarius with no cell bodies being detected. Calcitonin gene-related peptide-immunoreactivity was detected in nerve fibers in the nucleus tractus solitarius and cell bodies of the hypoglossal nucleus. Finally, neuropeptide Y-immunoreactivity was confined to nerve fibers within the vagal complex. Of the neurons labelled by the retrograde tracers injected into the corpus all were in close spatial contact with fibers containing substance P-immunoreactivity. A smaller number were associated with neuropeptide Y-containing fibers with a few adjacent to calcitonin gene-related peptide-immunoreactive fibers. These results indicate that substance P and neuropeptide Y may directly regulate efferent neurons controlling gastric motility and acid secretion. Calcitonin gene-related peptide, however, is unlikely to directly modulate the cell bodies of the neurons in the dorsal motor nucleus but may modulate the dendrites from these neurons in the nucleus tractus solitarius.
将逆行示踪剂注射到胃大弯处的胃后壁，导致迷走神经背运动核右半部分被标记。而注射到胃体前壁和后壁则导致延髓双侧被标记。使用P物质抗血清对标记切片进行免疫细胞化学染色，结果显示仅限于迷走神经背运动核和孤束核内密集的纤维网络，未检测到细胞体。在孤束核的神经纤维和舌下神经核的细胞体中检测到降钙素基因相关肽免疫反应性。最后，神经肽Y免疫反应性仅限于迷走复合体中的神经纤维。注射到胃体的逆行示踪剂标记的神经元中，所有神经元都与含有P物质免疫反应性的纤维紧密空间接触。较少数量的神经元与含有神经肽Y的纤维相关，少数与降钙素基因相关肽免疫反应性纤维相邻。这些结果表明，P物质和神经肽Y可能直接调节控制胃动力和胃酸分泌的传出神经元。然而，降钙素基因相关肽不太可能直接调节迷走神经背运动核中神经元的细胞体，但可能调节孤束核中这些神经元的树突。
