Faith Nancy G, Peterson Luke D, Luchansky John B, Czuprynski Charles J
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
J Food Prot. 2006 Nov;69(11):2664-70. doi: 10.4315/0362-028x-69.11.2664.
Although multistrain cocktails of Listeria monocytogenes are used in food inoculation experiments, no studies, to our knowledge, have been reported that use these cocktails in an intragastric mouse model. In this study, we used a five-strain L. monocytogenes cocktail consisting of strains Scott A, MFS108, 101M, V7, and 310 and a four-strain L. monocytogenes cocktail containing strains Scott A, EGD, H7738, and F2365. Here, we report that intragastric inoculation of anesthetized mice with approximately 106 CFU of a cocktail of L. monocytogenes strains does not result (P > 0.05) in a more severe infection (on the basis of the CFU of Listeria spp. recovered from the spleen, liver, and blood) than inoculation of mice with similar numbers of the individual strains comprising the cocktail. Nor did we observe any consistent relationship between susceptibility of L. monocytogenes strains to inactivation in synthetic gastric fluid in vitro and virulence in mice.
尽管在食品接种实验中使用了单核细胞增生李斯特菌的多菌株混合物，但据我们所知，尚无研究报道在小鼠胃内模型中使用这些混合物。在本研究中，我们使用了由斯科特A、MFS108、101M、V7和310菌株组成的五菌株单核细胞增生李斯特菌混合物，以及包含斯科特A、EGD、H7738和F2365菌株的四菌株单核细胞增生李斯特菌混合物。在此，我们报告，用约106 CFU的单核细胞增生李斯特菌菌株混合物对麻醉小鼠进行胃内接种，与用组成混合物的相似数量的单个菌株接种小鼠相比，不会导致更严重的感染（基于从脾脏、肝脏和血液中回收的李斯特菌属CFU）（P>0.05）。我们也未观察到单核细胞增生李斯特菌菌株在体外合成胃液中对失活的敏感性与在小鼠中的毒力之间存在任何一致的关系。
