Wiśniewska Katarzyna, Garbacz Katarzyna, Piechowicz Lidia
Katedra Mikrobiologii, Zakład Mikrobiologii Lekarskiej AM w Gdańsku.
Med Dosw Mikrobiol. 2006;58(2):113-7.
Sixty fenotypically coagulase-negative Staphylococcus aureus strains were screened for the presence of six adhesion genes. The strains were isolated from varied clinical samples and varied patients in 16 medical centers, in majority from the region of Gdansk. Multiplex PCR in two primer sets was used for detection of the following genes: bbp (bone binding protein), cna (collagen binding protein), ebp (elastin binding protein)and fnbB (fibronectin B binding protein), fib (fibrinogen bindng protein) and clfA (clunmping factor A). More than half (57%) of the examined population harbored four genes: fnbB,fib, cna i clfA. All of the strains were found to be clfA positive and 90% of them were positive for fnbB, 90% for fib and 67% for cna. All of these genes were significantly more common in MRSA than in MSSA. The particular genes were occurred in strains derived from varied clinical samples.
对60株表型凝固酶阴性金黄色葡萄球菌菌株进行了6种黏附基因的筛查。这些菌株从16个医疗中心的不同临床样本和不同患者中分离得到,大多数来自格但斯克地区。使用两组引物进行多重PCR检测以下基因:bbp(骨结合蛋白)、cna(胶原蛋白结合蛋白)、ebp(弹性蛋白结合蛋白)和fnbB(纤连蛋白B结合蛋白)、fib(纤维蛋白原结合蛋白)和clfA(聚集因子A)。超过一半(57%)的检测菌株携带4种基因:fnbB、fib、cna和clfA。所有菌株均被发现clfA呈阳性,其中90%的菌株fnbB呈阳性,90%的菌株fib呈阳性,67%的菌株cna呈阳性。所有这些基因在耐甲氧西林金黄色葡萄球菌(MRSA)中比在甲氧西林敏感金黄色葡萄球菌(MSSA)中更为常见。特定基因出现在来自不同临床样本的菌株中。