对从临床样本中分离出的非典型金黄色葡萄球菌菌株进行基因型筛查，以检测特定黏附素基因的存在情况。

Genotypic screening of atypical Staphylococcus aureus strains isolated from clinical samples for presence of selected adhesin genes.

作者信息

Wiśniewska K, Garbacz K, Piechowicz L

机构信息

Department of Medical Microbiology, Medical University of Gdańsk, 38, Do Studzienki Street, 80-227 Gdańsk, Poland.

出版信息

Med Mal Infect. 2008 Oct;38(10):549-53. doi: 10.1016/j.medmal.2008.06.003.

DOI:10.1016/j.medmal.2008.06.003

PMID:18672339

Abstract

OBJECTIVE

The aim of this study was to screen Staphylococcus aureus negative for production of coagulase or clumping factor and for presence of selected adhesin genes.

METHODS

Sixty coagulase-negative and 20 clumping factor-negative S. aureus strains were studied. Detection of methicillin resistance was performed using the agar screen technique with 6 mg/L of oxacillin and was confirmed by amplification mecA gene. The presence of bone binding protein (bbp), collagen binding protein (cna), fibronectin A binding protein (fnbA), fibronectin B binding protein (fnbB) and clumping factor A (clfA) genes was detected by multiplex PCR.

RESULTS

Almost all (98%) of the strains were positive for clfA gene. There were fnbA and fnibB in 85%, cna in 54% and bbp in 5% of strains found. No correlation between presence of the particular genes and clinical samples was observed. The prevalence of fnbA, fnbB and cna was statistically higher in coagulase-negative than in clumping factor-negative strains (89, 89, 66 and 70, 70, 15%, respectively). Similarly, all of these genes were more often observed in MRSA than in MSSA atypical strains. The cna was detected only in coagulase-negative MRSA.

摘要

目的

本研究旨在筛选凝固酶或聚集因子产生阴性且特定黏附素基因阴性的金黄色葡萄球菌。

方法

研究了60株凝固酶阴性和20株聚集因子阴性的金黄色葡萄球菌菌株。采用含6mg/L苯唑西林的琼脂筛选技术检测耐甲氧西林情况，并通过扩增mecA基因进行确认。通过多重PCR检测骨结合蛋白（bbp）、胶原蛋白结合蛋白（cna）、纤连蛋白A结合蛋白（fnbA）、纤连蛋白B结合蛋白（fnbB）和聚集因子A（clfA）基因的存在情况。

结果

几乎所有（98%）菌株的clfA基因呈阳性。在85%的菌株中发现有fnbA和fnibB，54%的菌株中有cna，5%的菌株中有bbp。未观察到特定基因的存在与临床样本之间的相关性。凝固酶阴性菌株中fnbA、fnbB和cna的流行率在统计学上高于聚集因子阴性菌株（分别为89%、89%、66%和70%、70%、15%）。同样，在耐甲氧西林金黄色葡萄球菌（MRSA）中比在甲氧西林敏感金黄色葡萄球菌（MSSA）非典型菌株中更常观察到所有这些基因。cna仅在凝固酶阴性的MRSA中检测到。