Ulrich A K, Li L Y, Parker J
Department of Microbiology, Southern Illinois University, Carbondale 62901.
Biochim Biophys Acta. 1991 Jul 23;1089(3):362-6. doi: 10.1016/0167-4781(91)90177-n.
The fidelity of codon reading was examined in amino acid starved Escherichia coli. In one case the level of misincorporation of methionine was measured at an isoleucine residue encoded by either the commonly used AUU codon or the rarely used AUA codon. In this situation we found the frequency of methionine misincorporation to be very low and to be unaffected by the identity of the isoleucine codon. In other experiments histidine misincorporation for glutamine was measured in glutamine starved cells with normal levels of histidine-specific tRNA and cells overproducing this tRNA. Cells overproducing the tRNA had higher levels of misincorporation.
在氨基酸饥饿的大肠杆菌中检测密码子阅读的保真度。在一个案例中，测量了在由常用的AUU密码子或罕用的AUA密码子编码的异亮氨酸残基处甲硫氨酸错掺入的水平。在这种情况下，我们发现甲硫氨酸错掺入的频率非常低，并且不受异亮氨酸密码子身份的影响。在其他实验中，在组氨酸特异性tRNA水平正常的谷氨酰胺饥饿细胞和过量产生这种tRNA的细胞中，测量了组氨酸错掺入为谷氨酰胺的情况。过量产生tRNA的细胞具有更高的错掺入水平。
