Imaki J, Imaki T, Vale W, Sawchenko P E
Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla, CA 92037.
Brain Res. 1991 Apr 26;547(1):28-36. doi: 10.1016/0006-8993(91)90571-c.
Hybridization histochemical and immunohistochemical methods were used to characterize the distribution of corticotropin-releasing factor (CRF) messenger RNA (mRNA) and peptide, respectively, in the central auditory system of the rat. Cell bodies expressing CRF mRNA and/or immunoreactivity (IR) were detected at each level of the system, including sparse or equivocal localizations in the dorsal cochlear nucleus, the medial nucleus of the trapezoid body, and the lateral superior olive. More prominent groups of cells expressing CRF mRNA and CRF-IR were found in the nuclei of the lateral lemniscus, the shell of the inferior colliculus, the medial division of the medial geniculate body and in primary auditory cortices. The latter showed the greatest density of CRF-expressing interneurons, distributed primarily in layers II, III and V, of any neocortical area. Results obtained using the two staining methods were in good agreement, except in the cochlear and superior olivary nuclei, where cells displaying CRF-IR were apparent in far greater abundance than those expressing CRF mRNA. CRF-IR fibers and terminals were detected in regions generally consistent with the cellular localizations described above. These results provide evidence for a surprisingly widespread expression of CRF in the auditory system of the rat. This includes generally low levels of expression in components of the primary auditory path (cochlear nucleus, trapezoid body, superior olive, lemniscal nuclei). CRF appears to be more prominently expressed in so-called non-primary components of the auditory system, including aspects of the medial geniculate body that constitute an interface between the auditory system and stress-related limbic system circuitry.
采用杂交组织化学和免疫组织化学方法，分别对大鼠中枢听觉系统中促肾上腺皮质激素释放因子（CRF）信使核糖核酸（mRNA）和肽的分布进行了表征。在该系统的各个层面均检测到了表达CRF mRNA和/或免疫反应性（IR）的细胞体，包括在蜗背侧核、斜方体内侧核和外侧上橄榄核中的稀疏或不明确定位。在外侧丘系核、下丘壳、内侧膝状体内侧部以及初级听觉皮层中发现了表达CRF mRNA和CRF-IR的更显著细胞群。在任何新皮层区域中，后者显示出表达CRF的中间神经元密度最高，主要分布在II、III和V层。除了在蜗核和上橄榄核中，两种染色方法得到的结果基本一致，在蜗核和上橄榄核中，显示CRF-IR的细胞明显比表达CRF mRNA的细胞丰富得多。在与上述细胞定位大致一致的区域检测到了CRF-IR纤维和终末。这些结果为CRF在大鼠听觉系统中惊人的广泛表达提供了证据。这包括在初级听觉通路（蜗核、斜方体、上橄榄核、丘系核）的组成部分中普遍较低的表达水平。CRF似乎在听觉系统的所谓非初级组成部分中表达更为突出，包括构成听觉系统与应激相关边缘系统回路之间界面的内侧膝状体的各个方面。
