Weeks Benjamin S, Perez Pedro P
Department of Biology and Environmental Sciences Program, Adelphi University, Garden City, NY, USA.
Med Sci Monit. 2006 Dec;12(12):BR379-384. Epub 2006 Nov 23.
Pesticide exposure is a recognized risk factor for neurodegenerative diseases. Recently, bifenthrin, a pyrethroid pesticide, was shown to inhibit the formation of neurites and cause neurite retraction, raising concern that these newer and less toxic pesticides may also contribute to neurodegenerative diseases. PolicosanolPlus and Neuroprevin are nutraceutical supplements which promote the survival of neurites in neuronal cell cultures. Here we determine if PolicosanolPlus and Neuroprevin can ameliorate the neurodegenerative effects of bifenthrin.
MATERIAL/METHODS: PC12 cells were treated with NGF, bifenthrin, PolicosanolPlus and Neuroprevin in various combinations and the formation of neurites was assessed microscopically at times ranging from 12 to 72 hours post treatment. Bifenthrin was also withheld at the time of NGF, PolicosanolPlus and Neuroprevin treatment and added after neurite formed to assess neurite retraction.
Bifenthrin (1 x 10(-6) M) inhibits neurite outgrowth, in the absence of cell death, by more than 50% at 12 hours and by more than 80% at 72 hours. With addition of PolicosanolPlus and/or Neuroprevin at the time of cell seeding, bifenthrin does not inhibit neurite outgrowth. Addition of bifenthrin to differentiated cells results in a retraction of 90% of neurites, while those with PolicosanolPlus and Neuroprevin show no significant retraction of neurites.
The pesticide, bifenthrin, inhibits neurite formation and causes neurite retraction. PolicosanolPlus and Neuroprevin are nutraceutical supplements which ameliorate the effects of bifenthrin on neurite outgrowth and retraction. Dietary supplementation with PolicosanolPlus and Neuroprevin may protect against developmental and long-term neurodegenerative events that result from exposure to pesticides.
接触农药是公认的神经退行性疾病风险因素。最近，拟除虫菊酯类农药联苯菊酯被证明可抑制神经突的形成并导致神经突回缩，这引发了人们对这些更新且毒性较低的农药可能也会导致神经退行性疾病的担忧。PolicosanolPlus和Neuroprevin是营养补充剂，可促进神经元细胞培养中神经突的存活。在此，我们确定PolicosanolPlus和Neuroprevin是否能改善联苯菊酯的神经退行性作用。
材料/方法：将PC12细胞用神经生长因子（NGF）、联苯菊酯、PolicosanolPlus和Neuroprevin以各种组合进行处理，并在处理后12至72小时的不同时间通过显微镜评估神经突的形成。在NGF、PolicosanolPlus和Neuroprevin处理时不添加联苯菊酯，待神经突形成后再添加以评估神经突回缩。
联苯菊酯（1×10⁻⁶ M）在无细胞死亡的情况下，12小时时抑制神经突生长超过50%，72小时时超过80%。在细胞接种时添加PolicosanolPlus和/或Neuroprevin，联苯菊酯不会抑制神经突生长。向分化细胞中添加联苯菊酯会导致90%的神经突回缩，而添加了PolicosanolPlus和Neuroprevin的细胞神经突无明显回缩。
农药联苯菊酯抑制神经突形成并导致神经突回缩。PolicosanolPlus和Neuroprevin是营养补充剂，可改善联苯菊酯对神经突生长和回缩的影响。补充PolicosanolPlus和Neuroprevin的饮食可能预防因接触农药导致的发育性和长期神经退行性事件。
