Abe R, Kanagawa O, Sheard M A, Malissen B, Foo-Phillips M
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
J Immunol. 1991 Aug 1;147(3):739-49.
In the mouse, two sets of V beta gene products have been shown to be associated with T cell recognition of endogenous self Ag. One of these is the set of V beta associated with T cell reactivities to stimulatory Mls gene products, Mlsa (V beta 6, V beta 8.1, V beta 9) or Mlsc (V beta 3); another is the set of V beta, such as V beta 5, V beta 11, V beta 12, or V beta 17a, which were originally found to be related to I-E recognition. Although the Mls system has been well characterized, little is known about the nature of the ligands for the second set of V beta. In this work, we describe the evidence that the natural ligand or ligands of V beta 5, V beta 11, and V beta 12 may be novel Mls determinants that are recognized by naive T cells at a high precursor frequency and function as the ligand for clonal deletion of self-reactive T cells by negative selection. However, surprisingly, unlike the conventional Mls system, in which all V beta associated with Mlsa recognition or Mlsc recognition are uniformly deleted in those animals expressing the relevant Mls type, expression of these three V beta segregates independently among strains. Based on these observations, the nature of T cell recognition for this new Mls gene product(s) is discussed.
在小鼠中，已证明两组Vβ基因产物与T细胞对内源性自身抗原的识别有关。其中一组是与T细胞对刺激性Mls基因产物Mlsa（Vβ6、Vβ8.1、Vβ9）或Mlsc（Vβ3）的反应性相关的Vβ；另一组是最初发现与I-E识别有关的Vβ，如Vβ5、Vβ11、Vβ12或Vβ17a。尽管Mls系统已得到充分表征，但对于第二组Vβ的配体性质知之甚少。在这项研究中，我们描述了证据表明，Vβ5、Vβ11和Vβ12的天然配体可能是新的Mls决定簇，它们以高前体频率被未成熟T细胞识别，并作为通过阴性选择克隆清除自身反应性T细胞的配体。然而，令人惊讶的是，与传统的Mls系统不同，在表达相关Mls类型的动物中，所有与Mlsa识别或Mlsc识别相关的Vβ都会被均匀删除，而这三种Vβ的表达在不同品系中是独立分离的。基于这些观察结果，讨论了T细胞对这种新的Mls基因产物的识别性质。
