亚慢性给予抗抑郁药对经对氯苯丙氨酸处理的大鼠海马切片中5-羟色胺刺激的磷酸肌醇水解的影响。

Effect of subchronic antidepressants administration on serotonin-stimulated phosphoinositide hydrolysis in para-chlorophenylalanine-treated rat hippocampal slices.

Kusumi I, Mikuni M, Takahashi K

Division of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan.

Prog Neuropsychopharmacol Biol Psychiatry. 1991;15(3):393-403. doi: 10.1016/0278-5846(91)90070-h.

Abstract

This study examines the effect of subchronic parachlorophenylalanine (PCPA) treatment upon serotonin (5-HT)-stimulated inositol monophosphate (IP-1) accumulation in rat hippocampal slices and also the effect of antidepressants upon this 5-HT response in the hippocampus from rats treated with or without concurrent administration of PCPA. 2. For high dose PCPA treatment, animals were injected intraperitoneally with 300 mg/kg daily for the first 5 days and then 100 mg/kg for 5 days, while for low dose PCPA treatment animals were injected for 10 days at a dose of 100 mg/kg. Imipramine or iprindole (15 mg/kg i.p.) was given once daily for 10 consecutive days. 3. 10-Day treatment with high dose of PCPA resulted in a significant increase in 5-HT-stimulated IP-1 accumulation, whereas low dose of PCPA had no significant effect upon the 5-HT response as compared to vehicle. 5-HT-stimulated IP-1 accumulation in rat hippocampus was not affected by subchronic treatment with imipramine or iprindole. The enhancement of the 5-HT response induced by high dose of PCPA was not attenuated by repeated antidepressants treatment.

摘要

本研究考察了亚慢性对氯苯丙氨酸（PCPA）处理对大鼠海马切片中5-羟色胺（5-HT）刺激的肌醇单磷酸（IP-1）积累的影响，以及抗抑郁药对接受或未同时给予PCPA处理的大鼠海马中这种5-HT反应的影响。2. 对于高剂量PCPA处理，动物在最初5天每天腹腔注射300 mg/kg，然后在接下来5天注射100 mg/kg；而对于低剂量PCPA处理，动物以100 mg/kg的剂量注射10天。丙咪嗪或茚满二酮（15 mg/kg腹腔注射）连续10天每天给药一次。3. 高剂量PCPA处理10天导致5-HT刺激的IP-1积累显著增加，而低剂量PCPA与赋形剂相比对5-HT反应无显著影响。大鼠海马中5-HT刺激的IP-1积累不受丙咪嗪或茚满二酮亚慢性处理的影响。高剂量PCPA诱导的5-HT反应增强未因重复给予抗抑郁药而减弱。