Grove Harald, Hollung Kristin, Uhlen Anne Kjersti, Martens Harald, Faergestad Ellen Mosleth
Matforsk AS, Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway.
J Proteome Res. 2006 Dec;5(12):3399-410. doi: 10.1021/pr0603250.
Assumptions that need to be considered prior to statistical analysis of protein spot volumes from two-dimensional gel electrophoresis (2-DE) data are studied using replicate gels of the same sample. The most important observation is that the data tables of protein spot volumes from 2-DE images contain a large number of missing values, which are not consistent with the presence or absence of the proteins. This implies both loss of information and problems for the subsequent statistical analysis. Challenges with 2-DE protein spot volumes are viewed in light of multiple gel comparisons and multivariate data analysis.
使用同一样品的重复凝胶，研究了在对二维凝胶电泳（2-DE）数据中的蛋白质斑点体积进行统计分析之前需要考虑的假设。最重要的观察结果是，来自2-DE图像的蛋白质斑点体积数据表包含大量缺失值，这些缺失值与蛋白质的存在与否不一致。这意味着信息丢失以及后续统计分析存在问题。从多次凝胶比较和多变量数据分析的角度来看，2-DE蛋白质斑点体积存在挑战。
