Højfeldt Jonas W, Blakskjaer Peter, Gothelf Kurt V
Center for Catalysis and Interdisciplinary Nanoscience Center (iNANO), Department of Chemistry, University of Aarhus, Langelandsgade 140, 8000 Aarhus C, Denmark.
J Org Chem. 2006 Dec 8;71(25):9556-9. doi: 10.1021/jo0619736.
A cleavable heterobifunctional cross-linker for the reversible conjugation of amines to thiol-modified DNA has been developed and tested. The succinimidyl 2-(vinylsulfonyl)ethyl carbonate (SVEC) was prepared in three steps and tested for its ability to react with amines and thiols. The linker was efficient for binding leucine to a thiol-modified DNA sequence and for releasing the amino acid at pH 11.8.
一种用于胺与硫醇修饰的DNA可逆偶联的可裂解异双功能交联剂已被开发并测试。碳酸2-(乙烯基磺酰基)乙酯琥珀酰亚胺酯(SVEC)分三步制备，并测试了其与胺和硫醇反应的能力。该交联剂能有效地将亮氨酸与硫醇修饰的DNA序列结合，并在pH 11.8时释放氨基酸。
