Worrall S, de Jersey J, Shanley B C, Wilce P A
Department of Biochemistry, University of Queensland, St Lucia, Australia.
Eur J Clin Invest. 1991 Feb;21(1):90-5. doi: 10.1111/j.1365-2362.1991.tb01364.x.
Several recent reports have shown that antibodies reactive with acetaldehyde (AcH)-modified epitopes are present in alcoholics. However, similar antibodies have also been found in patients with non-alcoholic liver disease and control subjects. In each of these studies total immunoglobulin binding to the AcH-modified proteins was measured, with no attempt being made to identify the classes of immunoglobulin involved. In the present study we employed an enzyme-linked immunosorbent assay (ELISA) to assess the classes of immunoglobulin involved in this response, using plasma samples from 97 alcoholics with varying degrees of liver disease, 35 patients with non-alcoholic liver disease and 33 control subjects. All three groups exhibited a large IgM response and a negligible IgG response. However, the alcoholics exhibited a significantly higher IgA response than either of the other groups. This suggests that the measurement of the IgA response to AcH-modified epitopes may be a specific marker of ethanol abuse.
最近的几份报告显示，与乙醛（AcH）修饰表位反应的抗体存在于酗酒者体内。然而，在非酒精性肝病患者和对照受试者中也发现了类似的抗体。在这些研究中，均测定了与AcH修饰蛋白结合的总免疫球蛋白，但未尝试鉴定所涉及的免疫球蛋白类别。在本研究中，我们采用酶联免疫吸附测定（ELISA）来评估参与该反应的免疫球蛋白类别，使用了97名患有不同程度肝病的酗酒者、35名非酒精性肝病患者和33名对照受试者的血浆样本。所有三组均表现出强烈的IgM反应和可忽略不计的IgG反应。然而，酗酒者的IgA反应明显高于其他两组中的任何一组。这表明，测量对AcH修饰表位的IgA反应可能是乙醇滥用的一个特异性标志物。
