Drímal J, Magna D, Knézl V, Tokárová J, Drímalová L
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava.
Gen Physiol Biophys. 1991 Apr;10(2):125-35.
Monolayer cultures of human embryonal smooth muscle cells (HEC) were used to study the heterologous regulation of membrane beta-adrenergic receptors and Ca2+ channels. The relationships between the activation of membrane bound alpha-1 and beta-adrenergic receptors, the cyclic nucleotide response and Ca2+ channel binding were studied in a cellular model of latent virus infection (Herpes simplex, Type-2) in a human embryonal cell line. In the early stage of infection (72 h), there was a significant increase in the cell cAMP content, followed by a decrease in the binding capacity of the beta-adrenergic ligand with an increased total number of the 1,4-dihydropyridine Ca2+ channel agonist (-)-S-(3H)BAYK 8644 binding sites on the cell membrane of infected cells. The increased numbers of Ca2+ agonist binding sites were accompanied by an increased cAMP content in the cells and an increased membrane ATP-ase activity. Down-regulation of (3H)DHA binding, and an increased capacity of Ca2+ agonist binding were found after prolonged exposure of HEC to isoprenaline (10(-5) mol.l-1). Stimulation of alpha-1 adrenergic receptors with phenylephrine (10(-6) mol.l-1) was accompanied with only slight but significant increase in (3H)DHA binding and with a significant reduction in the total number of Ca2+ channel agonist binding sites.
用人胚胎平滑肌细胞（HEC）的单层培养物来研究膜β-肾上腺素能受体和Ca2+通道的异源调节。在人胚胎细胞系的潜伏病毒感染（单纯疱疹病毒2型）细胞模型中，研究了膜结合的α-1和β-肾上腺素能受体的激活、环核苷酸反应和Ca2+通道结合之间的关系。在感染早期（72小时），细胞cAMP含量显著增加，随后β-肾上腺素能配体的结合能力下降，而感染细胞细胞膜上1,4-二氢吡啶Ca2+通道激动剂（-）-S-（3H）BAYK 8644结合位点的总数增加。Ca2+激动剂结合位点数量的增加伴随着细胞内cAMP含量的增加和膜ATP酶活性的增加。HEC长期暴露于异丙肾上腺素（10^-5 mol·l^-1）后，发现（3H）DHA结合下调，Ca2+激动剂结合能力增加。用去氧肾上腺素（10^-6 mol·l^-1）刺激α-1肾上腺素能受体，仅伴随着（3H）DHA结合略有但显著增加，以及Ca2+通道激动剂结合位点总数显著减少。
