Tassi C, Fortuna A, Bontadini A, Lemoli R M, Gobbi M, Tazzari P L
Instituto di Ematologia L. & A. Seragnoli, Universita di Bologna, Italy.
Haematologica. 1991 Mar;76 Suppl 1:41-3.
A column immunoadsorption method, based on the high affinity between the protein Avidin and the vitamin Biotin has been used to obtain positive CD34+ or S313+ marrow cells selection. Cell suspensions from marrow samples of six healthy subjects were incubated either the monoclonal antibody S313 or HPCA 1 (My 10-like) and a biotinilated goat anti-mouse Immunoglobulins antiserum, passed over a column containing an Avidin coated Polyacrylamide matrix, at the flow rate of 1 ml/min. The phenotype and the clonogenic efficiency of the recovered adherent cell fraction were studied by cytofluorimetric analysis and haemopoietic progenitors short term cultures. The results obtained show a mean CD34+ and S313+ cells recovery greater than 50% with a lower stem cells enrichment. Although these data could not considered optimal for clinical application in haematologic neoplasias, these preliminary studies demonstrate the possible use of the method for autologous bone marrow transplantation.
基于蛋白质抗生物素蛋白与维生素生物素之间的高亲和力，采用了一种柱免疫吸附方法来实现阳性CD34+或S313+骨髓细胞的分选。将来自6名健康受试者骨髓样本的细胞悬液与单克隆抗体S313或HPCA 1（My 10样）以及生物素化的山羊抗小鼠免疫球蛋白抗血清一起孵育，以1 ml/分钟的流速通过装有抗生物素蛋白包被的聚丙烯酰胺基质的柱。通过细胞荧光分析和造血祖细胞短期培养研究回收的贴壁细胞部分的表型和克隆形成效率。获得的结果显示，平均CD34+和S313+细胞回收率大于50%，但干细胞富集程度较低。尽管这些数据对于血液系统肿瘤的临床应用而言并非最优，但这些初步研究证明了该方法在自体骨髓移植中的可能用途。
